急性胆道梗阻大鼠肝细胞死亡方式的研究.doc

基础研究BASIC RESEARCH 急性胆道梗阻大鼠肝细胞死亡方式的研究 徐锋戴朝六贾昌俊许永庆彭松林顾玺赵阳 徐锋戴朝六贾昌俊许永庆彭松林顾玺赵阳中国医科大学盛京医院肝胆外科沈阳110004 徐锋，中国医科大学盛京医院肝胆外科通讯作者：中国医科大学盛京医院肝胆外科Study of Cell death patterns in rats with acute bile duct obstruction Feng XU, Chao-liu DAI, Chang-jun JIA, Yong-qing XU, Song-lin PENG, Xi GU, Yang ZHAO Feng XU, Chao-liu DAI, Chang-jun JIA, Yong-qing XU, Song-lin PENG, Xi GU, Yang ZHAO, Department of Hepatobiliary Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China Correspondence to: Doctor Feng Xu, Department of Hepatobiliary Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. xufeng_sy@163.com Received: Accepted: Abstract AIM: To investigate hepatic cell death patterns in rats with acute bile duct ligation. METHODS: Male Wistar rats were divided randomly into bile duct ligation group (BDL, n=5)　and control group (C, n=5). Rats of BDL group underwent ligation and division of common bile duct to set up acute bile duct obstruction model according to Yoshidome’s maneuver, but rats of C group only underwent dissociation of common bile duct. All animals were sacrificed on day 7. Flow cytometry was used to count the percentage of hepatocyte apoptosis and oncosis. HE stain and electron microscope were used to observe hepatocyte apoptosis, oncosis and histopathological change. An auto analyzer was used to determine serum total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), glutamyl transpeptidase (γ-GT), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels. RESULTS: The percentage of cell oncosis or necrosis was higher in BDL group than that in C group (oncosis: 25.41±2.18 vs 6.12±1.69; necrosis: 3.99±1.24 vs 0.79±0.29; all P0.05). The percentage of oncosis was higher than apoptosis in BDL group (25.41±2.18 vs 2.79±1.43). Hepatic histopathological change showed bile duct proliferation, fibrous connectiv