野西瓜总生物碱诱导HepG-2细胞凋亡机制初步研究.docVIP

Study on Preliminary Mechanism Apoptosis in HepG-2 by CSA Yu Lei1,2,3 Mo Ke2,3 Wang Wei2,3 Cui Rong-tian2,3 JI Yu-bin1,2,3 Zou Xiang1,2,3 (1.Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China; 2.Institute of Materia Medica and Postdoctoral Programme of Harbin University of Commerce, Harbin 150076, China;3.Engineering Reseach Center of Natural Anticancer Drugs,Ministry of Education) Abstract: Objective To study on the mechanism of killing and apoptosis inducing effect of total alkaloid in the CSA（Capparis spinosa L. alkaloid, CSA）on human heptocarcinoma cell Line HepG-2. Methods The killing effect of the CSA on human heptocarcinoma cell Line HepG-2 was measured by MTT method. Morphological observation of the HepG-2 cells was completed by fluorescence microscope. The apoptosis inducing effect and changing of mitochondria membrane potential of the CSA on the HepG-2 cells were measured by flowcytometry. In addition, effect of intracellular Ca2+ level of the CSA on the HepG-2 cells was studied by laser confocal microscope. Results The CSA has obvious cytotoxicity on the HepG-2 and seems to be dose-dependent, and its IC50 value is 162.4μg/mL. The HepG-2 cells have characteristic morphologic changes of apoptosis by the function of CSA, and the apoptosis percentage is higher than the natural one. The progress of cells cycle from S phase to G2 phase has been blocked, and the mitochondria membrane potential is markedly decreased ,and the intracellular Ca2+ level is increased by the function of CSA. Conclusion The CSA has obviously killing and apoptosis inducing effect on human heptocarcinoma cell Line HepG-2 by the mechanism of decreasing the mitochondria membrane potential and increasing the intracellular Ca2+ level. Keywords: CSA, Human Heptocarcinoma Cell Line HepG-2, Apoptosis, Mitochondrial Transmembrane Potential, Ca 2+ concentration 野西瓜总生物碱诱导HepG-2细胞凋亡机制的初步研究 于 蕾1,2,3 莫 科2,3