评价在囊型包虫病的免疫诊断抗原的纯化分数.pdfVIP

Parasitol Res DOI 10.1007/s00436-014-3947-0 ORIGINAL PAPER Evaluation of purified antigen fraction in the immunodiagnosis of cystic echinococcosis Katarína Reiterová Herbert Auer Nazmiye Altintaś Aysegul Yolasigmaz Received: 26 March 2014 /Accepted: 5 May 2014 # Springer-Verlag Berlin Heidelberg 2014 Abstract Echinococcus granulosus antigen B fraction (AgB) Introduction was evaluated for its prognostic value in the serological follow-up of cystic echinococcosis (CE), compared to crude Cystic echinococcosis (CE) is a serious zoonosis caused by hydatid fluid (HF) as well as soluble somatic Echinococcus larvae of tapeworms living as intestinal parasites of wild and multilocularis antigen (Em). The sensitivity and specificity of domestic carnivores and belonging to the genus Echinococcus enzyme-linked immunosorbent assays (ELISA) were com- (Craig et al. 1997) presenting an important public health issue pared examining 177 sera from patients with different clinical mainly in developing countries (Singh et al. 2013). Human courses and outcome of CE and with other parasitic infections. Echinococcus infections occur by accidental ingestion of eggs AgB-ELISA in comparison to confirmed cystic echinococco- excreted with the faeces of infected carnivores. Oncospheres sis has 96.4 % sensitivity and 97.2 % specificity, with 93.1 % hatch in the duodenum, penetrate the intestine and are positive predictive value and 98.6 % negative predictive val- transported via the bloodstream to various organs, mainly ue. The HF-ELISA was more sensitive than the AgB-ELISA, the liver (60–70 %), lungs (20–30 %), spleen, heart and brain. but its specificity was lower. Our results indicate that AgB- Because the symptoms vary according to the