物化实验-分光光度法测BPB电力平衡常数.pdf

BPB BPB 分光光度法测BBPPBB电力平衡常数 Authorandinstitution of thereport Authorandinstitution of thereport 【AAuutthhoorraannddiinnssttiittuuttiioonn oofftthheerreeppoorrtt】 Phangheng Phangheng PPBB1111220077002277zzhhaanngghheenngg School of Life Sciences. University of Science and Technology School of Life Sciences. University of Science and Technology SScchhooooll ooff LLiiffee SScciieenncceess.. UUnniivveerrssiittyy ooff SScciieennccee aanndd TTeecchhnnoollooggyy ofChina . HefeiCity.Anhui Province. ofChina . HefeiCity.Anhui Province. ooffCChhiinnaa .. HHeeffeeiiCCiittyy..AAnnhhuuiiPPrroovviinnccee.. Postcode:230026 Postcode:230026 PPoossttccooddee::223300002266 Email:zh322523@mail.ustc.edu.cn Email:zh322523@mail.ustc.edu.cn EEmmaaiill::zzhh332222552233@@mmaaiill..uussttcc..eedduu..ccnn Abstract Abstract 【AAbbssttrraacctt】 This experiment utilize spectrophotometer to find out the This experiment utilize spectrophotometer to find out the TThhiiss eexxppeerriimmeenntt uuttiilliizzee ssppeeccttrroopphhoottoommeetteerr ttoo ffiinndd oouutt tthhee maximum absorption wavelength of BPB, and then measure maximum absorption wavelength of BPB, and then measure mmaaxxiimmuumm aabbssoorrppttiioonn wwaavveelleennggtthh ooff BBPPBB,, aanndd tthheenn mmeeaassuurree absorbance in different pH, in order to calculate equilibrium absorbance in different pH, in order to calculate equilibrium aabbssoorrbbaannccee iinn ddiiffffeerreenntt ppHH,, iinn oorrddeerr ttoo ccaallccuullaattee eeqquuiilliibbrriiuumm constant of ionizationof BPB. constant of ionizationof BPB. ccoonnssttaannttooffiioonniizzaattiioonnooffBBPPBB.. Keywords Keywords 【KKeeyywwoorrddss】 Spectrophotometer Bromophenol blue Maximum absorption wavelength Absorbance The equilibrium constant of ionizati