慢病毒载体有效转移并感染心肌成纤维细胞_方欣.pdf

13 11 Journal of Clinical Rehabilitative Tissue Engineering Research March 12, 2009 Vol.13, No.11 * Transduction and infection of cardiac fibroblasts by lentivirus vector Fang Xin, Guan Si-ming, Li Wei, Wang Bin Abstract BACKGROUND: The pathological basis of cardiac fibrosis is proliferation of cardiac fibroblasts and increase of collage synthesis Department of of the cells. Lentivirus vector is a new gene-transfer system, which can infect split cells and unspilt cells effectively. Geriatrics, Affiliated OBJECTIVE: To investigate the efficient and stable transduction of cardiac fibroblasts by lentivirus vector, which can provide a Xiehe Hospital of Tongji Medical basis for cardiac fibroblasts gene-targeted therapy. College, Huazhong DESIGN, TIME AND SETTING: A contrast observation was performed at the Department of Geriatrics, Affiliated Xiehe Hospital University of Science of Tongji Medical College, Huazhong University of Science and Technology and the Laboratory of Shanghai GeneChem Co., Ltd and Technology, from August to October 2008. Wuhan 430022, Hubei Province, MATERIALS: Thirty new-born Sprague Dawley rats with 13 days old. Lentivirus vector, Enhanced Infection Solution (ENi.S), China and Polybrene was provided by Shanghai GeneChem Co., Ltd. METHODS: Cardiac fibroblasts of newborn rats were isolated and cultured with the technique of differential anchoring velocity, Fang Xin , Studying Lentivirus infected cells with three gradients were added into DMEM, three groups of cells with multiply of infection (MOI) of 200, for doctorate,