毛细管电泳-ESI芯片教案.pdfVIP

Anal. Chem. 2007, 79, Fully Microfabricated and Integrated SU-8-Based Capillary Electrophoresis-Electrospray Ionization Microchips for Mass Spectrometry Tiina Sikanen,†,‡ Santeri Tuomikoski,§ Raimo A. Ketola,‡,⊥ Risto Kostiainen,‡ Sami Franssila,§ and Tapio Kotiaho*,†,‡ Laboratory of Analytical Chemistry, Department of Chemistry, P.O. Box 55, FI-00014 University of Helsinki, Finland, Division of Pharmaceutical Chemistry, Faculty of Pharmacy, P.O. Box 56, FI-00014 University of Helsinki, Finland, Micro and Nanosciences Laboratory, Department of Electrical and Communications Engineering, P.O. Box 3500, FI-02015 Helsinki University of Technology, Finland, and Drug Discovery and Development Technology Center, P.O. Box 56, FI-00014 University of Helsinki, Finland We present a fully microfabricated and monolithically for bioanalysis. In recent years, numerous chip-based designs46 integrated capillary electrophoresis (CE)-electrospray ion- have been presented for miniaturization of the ESI source in order ization (ESI) chip for coupling with high-throughput mass to reach more efficient ionization through nanospray.7,8 Most spectrometric (MS) analysis. The chips are fabricated fully importantly, nanospray ionization provides not only increased of a negative photoresist SU-8 by a standard lithographic sensitivity but also direct coupling with microfluidic separation process which enables straightforward batch fabrication devices due to congruent flow rates between the two. of multiple chips with precisely controlled dimensions Chip-based ESI sources have been implemented on various and, thus, reproducible analytical performance from chip materials, including silicon, glass, and polymers.46 Dependi