高效液相色谱峰压缩中灵敏度增加值与峰压缩因子的关系研究.pdfVIP

Vo l. 28 No. 1 分析测试学报 第28 卷第l 期 FE II XI CESHI XlEBAO( Journal f In ,lrumplIlal Anal)sís) 18-21 2∞9 年 I fI 高效液相色谱峰压缩中灵敏度增加值与 峰压缩因子的关系研究 魏玉霞，李丽莉，才德，肖盛元，朱勇，邓玉林 (北京理工大学生命科学与技术学院，北京 10∞81 ) 摘 要:以从塔饭理论得到的色谱流出曲线为基础，推导了高效i夜相色谱( HPI.C) 双流动相分离场中实现峰 压缩时，峰压缩同手(峰JI~缩后的半崎宽与未进行峰压缩时的半峰宽的比值)和峰高表示的灵敏度变化(峰压 缩后的峰高与未注行峰压缩时的峰高的比值)的关系，结果表明峰高表示的灵敏度增加值等于峰压缩间子的 倒数。并用色氨酸、杏豆素、苯和2-硝基酣在本l司速率主实现堆积时的相关参数进行了验证。实验结果表 明，这些化合物在佟个峰压缩条件下的峰压缩回子倒数和实际灵敏度增加侦相等，实验结果与理论推导结果 一致c 考察了该关系式在实际样品测定中的应用，结果表明灵敏度增加值等于峰压缩回子的倒数，这也与理 论部分的结果-致 关键词:高敛液相i 色i普;灵敏度增加值;峰，，~缩回F 中图分类号: 0657.72 文献标识码:A 文章编号: 1ω4 -4957(2ω9)01 -∞18 -04 Assessment of Relationship between Peak Compression Factor and Sensitivity Enhancement Factor in HPLC Peak Compression WEI Yu-xia , LI Li-li , CAI I)e , XIAO Sheng-yuan , ZHU Yong , DENG Yu-lin (~hool of Life Science Techno!ogy. Beijing Institute of Teehno!ogy. Beijing 1 仪则81. China) Abstract: ßased on the chromatographic elution curve from the plate theory , the relationship be- tween the peak compression factor and the sensitivity enhancement factor was investigated when the peak was compressed in HPLC dual mohile phase separalion field. The result showed Ihal Ihe sensi- tivity improvemenl factor was equal to the reciprocal of the peak compression factor. The relalionship was tested by using tryptophan , coumarin , benzene and 2-nitrophenol as model , which indicated Ihat the experimental results malched well wilh Ihe Iheorelical prediction. The peak compression fac- tor and sensitivity enhancement factor of Ihe analyte in real sa