苍术提取物对脾虚证大鼠胃粘膜和胃肠免疫功能的影响.PDFVIP

苍术提取物对脾虚证大鼠胃粘膜及胃肠免疫功能的影响苍术提取物对脾虚证大鼠胃粘膜及胃肠免疫功能的影响 1，2 2 1 刘 芬 ，刘艳菊 ，田春漫 1 2 湖北民族学院，湖北 恩施 445000 ；湖北中医药大学，湖北 武汉 430065 摘要：目的探讨苍术提取物（Rhizome Atractylodes extract, ERA）保护胃粘膜的作用及对脾虚模型大鼠胃肠免疫功能的影响， 阐明ERA 干预脾虚证的作用机制。方法 采用喂饲小承气汤煎剂加饥饱失常建立大鼠脾虚证模型，模型复制成功后动物随机 分为脾虚模型组，ERA 高、中、低剂量组，多潘立酮组。连续灌胃给药10 d。采用肠道灌流法检测大鼠肠道灌流液IgA 含量，腹 主动脉采血法检测大鼠血清IgG 含量，并测定大鼠胸腺、脾脏指数，HE 染色行大鼠胃黏膜病理学观察，激光多普勒微循环血流 计行大鼠胃黏膜血流量的测定，免疫组化法测定大鼠胃黏膜组织中TFF 1及结肠TLR4 的表达量。结果 与正常组比较，模型组 大鼠胃黏膜形态学、胃黏膜血流量、相关免疫学指标显著改变；与模型组比较，ERA 各剂量组大鼠胃黏膜形态学、胃黏膜血流量、 胃黏膜组织中三叶因子1（TFF 1）的表达量，肠道灌流液IgA、血清IgG 含量、胸腺、脾脏指数及结肠TLR4 的表达量不同程度升 高，差异均有统计学意义（P0.05 或P0.01）。结论 ERA 可抑制脾虚证大鼠胃粘膜损害，保护和修复损伤的粘膜组织，并改善 脾虚证大鼠的免疫功能。 关键词：苍术提取物；脾虚证；胃粘膜；免疫功能 Effect of Rhizoma Atractylodis extract in protecting gastric mucosa and modulating gastrointestinal immune function in a rat model of spleen deficiency 1, 2 2 1 LIU Fen , LIU Yangju , TIAN Chunman 1Hubei Institute for Nationalities, Enshi 445000, China; 2 College of Pharmacy of Hubei University of Chinese Medicine, Wuhan 430065, China Abstract: Objective To investigate the effect of Rhizoma Atractylodis extract (ERA) in protecting gastric mucosa and modulating gastrointestinal immune function of a rat model of spleen deficiency syndrome and elucidate the mechanism by which ERA improves spleen deficiency syndrome. Methods Male rats fed with Xiaochengqi decoction and subjected to irregular feeding to induce spleen deficiency syndrome. The established models were randomized into model group, high-, moderate- and low-dose ERA groups, and domperidone group. After corresponding treatment for 30 days, the content of IgA in the intestinal lavage fluid, serum IgG, and the indices of the spleen and thymus were determined. The pathological changes in the gastric muco