DMA增加正常大鼠心肌细胞钙瞬变及收缩_英文_.pdfVIP

Acta Physiologica Sinica 　219 　 J un. 2002 , 54 (3) , 219～224 Research Paper 　　 2 + Enhancement of Ca transients and contraction of single ventricular myocytes of rats by 5( N, Ndimethyl) amiloride CU I XiangLi , CHEN HuanZhen , WU DongMei , WU BoWei Depart ment of Physiology , S hanxi Medical U niversity , Taiyuan 030001 ( ) ( ) ( + + + 2 + Abstract : 　 The effects of 5 N ,Ndimethyl amiloride DMA a blocker of Na / H exchanger or Na / Ca ex changer) on calcium transient and cell contraction in isolated ventricular myocytes in normal rats and rats with myocardial hypertrophy were examined using ion imaging system with a charge coupled digital camera (CCD camera) . Loading my 2 + ocytes with Fura2 , electrically triggered Ca transients and cell shortening were measured simultaneously. The results 2 + μ μ showed that 10 mol/ L DMA increased Ca transient and cell shortening from 209. 60 ±54. 96 and 3. 07 ±0. 97 m to μ ( ) 238. 50 ±80. 41 and 4. 07 ±1. 02 m , respectively P 0. 05 , which was completely abolished by application of KB + 2 + 2 + R7943 , a specific reverse