UCP1蛋白及UCP3基因表达减少及OLETF鼠早期肥胖有关.pdfVIP

2011 3 March 2011 31 3 Basic ClinicalMedicine Vol. 31 No. 3 : 2011) UCP1 UCP3 OLETF 1* 1 1 2 金成吉 , 王晓梅, 李 湘, 母义明 ( 1 , 116001; 2 , 100039) : 2 OLETF(UCPs) OLETF LETO, 710 25 W estern blot ( BAT) UCP1, Northern blot UCP2 UCP3 RNA OLETF LETO, 910 LETO UCP1 OLETF 19 (P 005); UCP3 RNA OLETF 55(P 001) UCP1 UCP3 OLETF : ; UCP1; UCP2; UCP 3 : R 5892 : A UCP1 and UCP3 play an i portant role in the early stage of the develop ent of obesity inOLETF rats 1* 1 1 2 JIN Chengji , WANG Xiao ei, LIXiang, MU Y i ing ( 1Dept. ofEndocrinology, theZhongshanHospitalAffiliated to DalianUnivesity, Dalian 116001; 2Chinese PLA GeneralHospital, Beijing 100039, China) Abstract: Objective To investigate the relationship between thebodyweightchange and the RNA or protein ex pression of uncoupling proteins (UCPs) in OLETF rats.M ethods Bodyweightwas easuredweekly. UCP1 pro tein expression in brown adipose tissuewas detected byWestern blot, whileUCP2 and UCP3 RNA expression in skeletal usclewas easured byNorthern hybridization. R esults OLETF rats gainedweightw ith a faster rate than LETO rats, and the difference in the rate ofweightgain was ostpro inent around 9 weeks ofage. UCP1 protein levelwas 19fold higher in LETO thanOLETF rats at 10weeks of age (P 005). UCP3 RNA levelwas55 fold higher in LETO than OLETF rats at 10 weeks of age (P 001). Conclusion UCP1 and UCP3 expression