维持胚胎干细胞多能性及自我更新的转录因子.pdf

HEREDITAS (Beijing) 2008 5 , 30(5): 529―536 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2008.00529 Oct-4/ Nanog 周一叶, 曾凡一 , 200040 : Oct-4 和Nanog 是两种维持干细胞多能性和自我更新的转录因子, 它们通过结合靶基因调控区, 选择性地 抑制分化基因表达或促进多能性基因表达。它们通常只在多能干细胞中表达, 在分化细胞中不表达。在不同的 发育阶段, 它们的表达量受到特异调控, 并且分别与 Sox-2 、FoxD3 等其他转录因子以及 LIF 、BMP 等胞外信 号通路互相作用, 形成一个复杂的转录调节 crosstalk 网络, 在特异时空激活或抑制靶基因的转录; 通过互相制 约最终决定干细胞是保持多能性还是分化, 以及向哪个方向分化。此外, Oct-4 和Nanog 对体细胞重编程为多能 细胞也有重要作用。 : Oct-4; Nanog; 多能性; 自我更新; 分化 Two vital transcriptional factors Oct-4 and Nanog to keep the pluripotency and self-renewal of stem cells and related regulation network ZHOU Yi-Ye, ZENG Fan-Yi Shanghai Institute of Medical Genetics, Shanghai Jiao Tong University, Shanghai 200040, China Abstract: Oct-4 and Nanog are two critical transcriptional factors to keep pluripotency and self-renewal of stem cells in vivo and in vitro, and they usually express only in pluripotent cells and not in differentiated cells. They bind to the regula- tory regions of targeted gene and often interact with other transcriptional factors and extracellular signal path components, such as Sox-2, FoxD3, LIF and BMP in specific tissues or developmental stages. So that all of these constitute a transcrip- tional crosstalk, and finally determine the cells destiny: keeping pluripotency or turning to differentiation. Keywords: Oct-4; Nanog; pluripotent; self-renewal; differentiation 1 Oct-4 Nanog Oct-4 , [1] Chambers IMitsui K , [2] [3] EScDNA ;