消酐散对肾气虚模型大鼠肾组织AQP2表达的影响_王德山.pdfVIP

2010 1 11 1 　CJITWN, January 2010, Vol.11, No.1　　　　　　　　　　　　　　　　　　　　　　　　 ·25 · AQP2 ＊ ① ① ① ① 王德山 　王　哲 　太史春 　王道平 　　〔　〕　:探讨消酐散对肾气虚模型大鼠肾功能、肾脏集合管上皮主 胞水通道蛋白 2(aquaporin2, AQP2)表达变 化。:应用 丁胺卡那霉素复制肾气虚模型, 采用消酐散治疗, 于 21 d 后检测肾功能, 采用免疫组化、Western blot 方法、RT -PCR 方法测定肾组织AQP2 蛋白及mRNA 表达的变化。:消酐散治疗组大鼠肾功能与模型组比较明显改善(P 0.05);AQP2 的表达显著上调(P0.01)。:消酐散具有明显的改善肾气虚模型大鼠肾功能的作用;其机制可能与上调 肾气虚模型大鼠肾小管上皮 胞AQP2 表达有关。 〔〕　消酐散　肾气虚模型　水通道蛋白-2　肾功能 Effect of Xiaogansan on Renal Aquaporin-2 Expressionin in Kidney-Qi Deficiency Model Rats WAN G Deshan , WAN G Z he, TAI Shichun, et al L iaoning Un iversi ty of T CM , S heny ang (110032) 　　ABSTRACT　O jective:To study t e effects of Xiaogansan on renal function, endot elin(ET )and aquaporin-2 (AQP2)ex- pression of c ief cell on kidney collecting duct in kidney-qi deficiency model rats.Methods:T e rats of kidney-qi deficiency model made by injecting amikacin were treated by Xiaogansan for 21 days.T en renal function w as detected, and plasma ET was measured wit radioimmunoassay.And t e expression of AQP2 protein and its mRNA in kidney tissues were detected wit immuno istoc e- mistry, Western blot analysis and RT-PCR.Results:Compared wit t ose in t e rats of t e model group, t e renal function w as improved markedly(P0.05);and AQP2 expression was up-regulated obviously;and plasma ET concentration was obviously lower in t e Xiaogansan treatment group (P0.01).Conclusion:Xiaogansan can improve t e renal function of kidney-qi deficien- cy model rats and its mec anism is related to up-regulation of AQP2 expression of t e kidney tissue and in ibition of ET secretion. KEY　WORDS　Xiaogansan　Model of kidney-qi deficiency　Aquaporin-2　Renal function 　　, 。: 、 , ,24