ADS-7多功能吸附树脂制备高纯度丹酚酸B的应用研究.PDF

ADS-7 多功能吸附树脂制备高纯度丹酚酸B 的应用研究 陶华明1 1 2 ，朱全红 ，徐满才 (1.南方医科大学中医药学院，广州 510515 ；2.湖南师范大学化学化工学院，长沙 410081) 摘要：目的 探讨ADS-7 多功能吸附树脂对丹参中丹酚酸B 的分离纯化方法。方法 以丹酚酸B 的含量及洗脱率为指标， 通过动态吸附试验考察ADS-7 树脂的吸附性能。结果 ADS-7 树脂对丹参中丹酚酸B 具有较高的吸附选择性，吸附量为 95.7 mg·g1(干树脂) ，甲酸/50% 乙醇(10 ∶90)作为洗脱剂，丹酚酸B 的纯度高达87.2%。结论 ADS-7 树脂用于丹参中丹 酚酸B 的分离纯化简单高效。 关键词：丹参；丹酚酸B ；ADS-7 树脂；纯化 中图分类号：R284.2 文献标志码：B 文章编号：1007-7693(2012)02-0113-04 Preparation of High-purity Salvianolic Acid B from Salvia Miltiorrhiza with Multifunctional Resin ADS-7 1 1 2 TAO Huaming , ZHU Quanhong , XU Mancai (1.School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; 2.College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China) ABSTRACT: OBJECTIVE To study the method for separating and purifying the salvianolic acid B from Salvia miltiorrhiza with multifunctional resin ADS-7. METHODS By using the content and elution rate of salvianolic acid B as indexes, the dynamic adsorption tests were employed to investigate adsorption capability of ADS-7 resin. RESULTS ADS-7 resin could be used to produce the salvianolic acid B with high quality. The dynamic adsorption capacity was 95.7 mg·g1 (dry resin), the purity of the salvianolic acid B was 87.2% when eluted with formic acid/50% ethanol (10 ∶90). CONCLUSION It is a simple and efficient method to separate the salvianolic acid B from Salvia miltiorrhiza with ADS-7 resin. KEY WORDS: Salvia miltiorrhiza; salvianolic acid B; ADS-7 resin; purification 丹参(Salviae Miltiorrhizae Radix)为双子叶植 差异进行物理吸附分离，对于其他与丹酚酸B极性 物唇形科鼠尾草属植物丹参(Salvia miltiorrhiza 相近的杂质则无法实现选择性分离。本实验根据丹 Bge.) 的干燥根及根茎，是我国传统医药学中应用 酚酸B分子结构特点，选用ADS-7 多功能吸附树脂 最早而且最广泛的活血化瘀药之一。水溶性丹参 对丹参水提取物进行吸附和解吸，发现