欧洲药典2.2.24.pdf

2.2.24. Absorption spectrophotometry, infrared EUROPEAN PHARMACOPOEIA 8.0 Operate an atomic absorption spectrometer in accordance The calibration curve is calculated by least-square regression with the manufacturer’s instructions at the prescribed from all measured data. The regression curve, the means, the wavelength. Introduce a blank solution into the atomic measured data and the confidence interval of the calibration generator and adjust the instrument reading so that it indicates curve are plotted. The operating method is valid when: maximum transmission. The blank value may be determined – the correlation coefficient is at least 0.99, by using solvent to zero the apparatus. Introduce the most – the residuals of each calibration level are randomly concentrated reference solution and adjust the sensitivity to distributed around the calibration curve. obtain a maximum absorbance reading. Rinse in order to Calculate the mean and relative standard deviation for the avoid contamination and memory effects. After completing the analysis, rinse with water R or acidified water. lowest and highest calibration level. When the ratio of the estimated standard deviation of the If a solid sampling technique is applied, full details of the lowest and the highest calibration level is less than 0.5 or procedure are provided in the monograph. greater than 2.0, a more precise estimation of the calibration Ensure that the concentrations to be determined fall preferably curve may be obtained using weighted linear regression. Both within the linear part of the calibration curve. If this is not linear and quadratic weighting functions are applied to the possible,