《生物信息学》练习题.docVIP

在Genbank中查找以下6个植物蛋白序列：protein1:NP_974673.2; protein2: NP_187969.1; protein3: NP_190855.1; protein4: NP_565618.1; protein5: NP_200511.1; protein6: NP_191407.1 (以FASTA格式)。 用EBI上的ClustalW2工具对其进行多序列比对，分析各蛋白序列之间的同源性。 比对结果表明：protein1:NP_974673.2和protein4: NP_565618.1的亲缘关系最近。 利用Phylip软件，选择距离法构建其进化树（要求写出具体的建树步骤）。 任意选取其中的一个蛋白进行蛋白质一级序列分析、二级结构预测及三维结构的模拟。 选择protein3: NP_190855.1 一级结构 网址：/tools/protparam.html Number of amino acids: 456 氨基酸数目 Molecular weight: 51154.5 相对分子质量 Theoretical pI: 8.69 理论 pI 值 Amino acid composition 氨基酸组成 Ala (A) 30 6.6% Arg (R) 28 6.1% Asn (N) 15 3.3% Asp (D) 27 5.9% Cys (C) 5 1.1% Gln (Q) 18 3.9% Glu (E) 28 6.1% Gly (G) 37 8.1% His (H) 16 3.5% Ile (I) 16 3.5% Leu (L) 42 9.2% Lys (K) 32 7.0% Met (M) 5 1.1% Phe (F) 17 3.7% Pro (P) 16 3.5% Ser (S) 46 10.1% Thr (T) 21 4.6% Trp (W) 8 1.8% Tyr (Y) 19 4.2% Val (V) 30 6.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0% 正/负电荷残基数 Total number of negatively charged residues (Asp + Glu): 55 Total number of positively charged residues (Arg + Lys): 60 Atomic composition: 原子组成 Carbon C 2270 Hydrogen H 3531 Nitrogen N 645 Oxygen O 686 Sulfur S 10 Formula: C2270H3531N645O686S10 分子式 Total number of atoms: 7142 总原子数 Extinction coefficients: 消光系数 Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 72560 Abs 0.1% (=1 g/l) 1.418, assuming all pairs of Cys residues form cystines Ext. coefficient 72310 Abs 0.1% (=1 g/l) 1.414, assuming all Cys residues are reduced Estimated half-life: 半衰期 The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). 20 hours (yeast, in vivo).