拟南芥叶绿体分裂突变体cpd44中ARC6基因的鉴定与.pdfVIP

植物生理学报 Plant Physiology Journal 2015, 51 (4): 501~508　　doi: 10.13592/ki.ppj.2015.0056 501 cpd44 ARC6 拟南芥叶绿体分裂突变体 中 基因的鉴定与分析 张琴, 贾宁, 李慧, 高宏波* 北京林业大学生物科学与技术学院, 北京100083 : , EMS (ethyl 摘要 叶绿体是光合作用的主要场所 叶绿体的分裂对于维持其正常的功能有着非常重要的作用。本实验从 methane sulfonate)诱变的拟南芥Columbia生态型(Col)种子中筛选到一个叶绿体分裂突变体cpd44 (chlorop last division 44), , 1~5 其叶绿体的体积显著增大 每个叶肉细胞中的叶绿体数目减少至 个。遗传分析显示该表型是单基因控制的隐性突变。 ARC6 (accumulation and replication of chloroplasts 6) , 1 160 C 运用图位克隆技术和测序分析发现 基因发生突变 第 个碱基由 T, RT-PCR cpd44 ARC6 mRNA 突变成 发生无义突变。半定量 分析结果显示 中 的 可能被降解。遗传互补实验进一步证明 cpd44 ARC6 的叶绿体异常表型是由 突变造成的。本工作为进一步研究叶绿体分裂机制提供了新材料。 : ; ; ARC6 ; cpd44 关键词 拟南芥 叶绿体分裂 Identification and Analysis of ARC6 Gene in a Chloroplast Division Mutant cpd44 in Arabidopsis thaliana ZHANG Qin, JIA Ning, LI Hui, GAO Hong-Bo∗ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China Abstract: Chloroplasts are the major place for photosynthesis. The division of chloroplast is important for the maintaining of its function. chlorop last division 44 (cpd44) is a chloroplast division mutant obtained in a mu- tant screening of ethyl methane sulfonate (EMS) mutagenized Arabidopsis thaliana in Col ecotype, in which the chloroplast volume was increased obviously and the chloroplast number was decreased to one to five per mesophyll cell. Genetic analysis indicated that the phenotype of cpd44 was controlled by a single recessive gene. Map-based cloning and sequencing analysis revealed a mutation in accumulation and replication