DNA分子标记技术在乳酸菌多态性研究中地应用.PDF

微生物学通报 AUG 20, 2008, 35(8): 1297~1301 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 专论与综述 DNA 分子标记技术在乳酸菌多态性研究中的应用 剧 柠 靳 烨* ( 010018) 摘 要: 本文概述了常用于乳酸菌分离鉴定及多态性研究中的基于 rDNA 序列的分子标记技术和 几种DNA 指纹图谱技术(RAPD, ARDRA, AFLP, REP/ERIC-PCR), 并对这些技术的原理、方法及 其近几年在乳酸菌研究中的进展进行了介绍。同时本文还比较了各种方法的优缺点, 不同的研究 方法, 其分辨率和检测限不同, 必须根据研究目的, 选择合适的分析方法。 关键词: 乳酸菌, DNA 标记技术, 分类, 鉴定, 多样性 The Application of Molecular Marking Technology in Diversity Research of Lactic Acid Bacteria * JU Ning JIN Ye (Inner Mongolia Agricultural University , Hohhot 010018) Abstract: This paper outlines some molecular marking technology based on rDNA sequences and several DNA fingerprinting technology (RAPD, ARDRA, AFLP, REP/ERIC-PCR) used in classification, identifica- tion and diversity research in lactic acid bacteria. The principles, methods and progress in recent years of these technologies were also introduced. At the same time, this paper also compares the advantages and dis- advantages of these methods. People should choose suitable method according to their purposes. Keywords: Lactic acid bacteria, DNA marking technology, Classification, Identification, Diversity DNA 1 基于rDNA 序列的分子标记技术 1.1 16S rDNA 序列同源性分析 r R N A 3 , 23S rRNA16S rRNA5S rRNA,