仔猪黄白痢大肠杆菌耐药性检测及ERIC图谱_陈俭要点分析.pdf

36(2):125 ～132, 2010 浙江大学学报(农业与生命科学版)　 Journal of Zhejiang University (Agric.Life Sci.) :1008-9209 (2010)02-0 125-08 DOI :10.3785/j.issn.1008-9209.2010.02.002 ERIC 陈俭, 姜中其, 唐一鸣 (浙江大学动物科学学院, 浙江 杭州310029) 　:以47 株黄白痢临床分离大肠杆菌为材料, 通过Kirby-Bauer 法检测细菌耐 表型并用PCR 扩增Ⅰ 型整合酶基因, 进而对不同耐 谱菌株进行以肠杆菌科基因间重复 一致序列为引物的聚合酶链反应 (ERIC-PCR)基因分型并用统计分析软件SPSS 16.0 聚类.耐 表型检测结果显示:47 株大肠杆菌均呈多 重耐 , 共9 种耐 表型Ⅰ;型整合子阳性菌株检出率为 95.7%(45/47);ERIC-PCR 扩增出现稳定可重复 的基因指纹图谱, 耐9 ～13 种 物菌株(85.1%, 40/47)的ERIC 指纹图谱分别显示出2 种主要谱型, 各代 表 1 个猪场的菌株类型.聚类分析提示, 相同耐 谱的来自同一猪场和不同猪场分离株的平均相似率分别 为68.7%和53.5%,显著高于47 株菌的平均相似率37.3%.说明菌株耐 性可能与特定的ERIC 指纹图 谱相关, ERIC 指纹图谱分析可作为考察猪源致病性大肠杆菌多重耐 表型与基因型特征的新视角. 　　:大肠杆菌;耐 表型;肠杆菌科基因间重复一致(ERIC)序列;聚类分析 :Q 933 ;S 852.6 　　　:A CH EN Jian, JIA NG Zh ng-qi, TA NG Yi-ming (College of Animal Sciences, Zhej iang University , H angzhou 310029, China) Antimicrobial resistance of Es cherichia coli from piglets with pre-weaning iarrhea an analysis of its enterobacterial repetitive intergenic consensus fingerprinting profiles.J urnal f Zhejiang University (Agric.Life Sci.), 20 10, 36 (2):125-132 Abstract :In rder t investigate the relati nship between fingerprinting and antimicr bial resistance patterns, 47 is lates f Escherichiacoli fr m piglets with pre-weaning diarrhea fr m 2 l cal large piggeries were c llected f r susceptibility testing by Kirby-Bauer meth d and studied the presence f integr n integrase gene by p lymerase chain reacti n (PCR), respectively.The is lates with different phen types were then gen typed by enter bacterial repetitive intergenic c nsensus (ERIC)-PCR fingerprinting and further clustered n the basis f the pr files using statistical s ftware SPSS 16.0.The results sh wed that the 47 is late