上海市内不同水质地河道春季浮游细菌群落结构分析.PDFVIP

微生物学通报 APR 20, 2009, 36(4): 522~527 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 上海市内不同水质的河道春季浮游 细菌群落结构分析 * 姜彩虹 张美玲 陶琰洁 禹 娜 陈立侨 ( 200062) 摘 要: 2008 年4 月采集上海市城郊结合地区的4 条河道午潮港、横港、朝阳河和曹杨环浜的水 样, 测定水样的相关理化数据, 计算水环境质量综合指数; 提取水样中浮游细菌的总 DNA, 进行 变性梯度凝胶电泳(DGGE)分析; 并对水体中的可培养浮游细菌的数量进行测定。结果表明, 污染 严重的河道浮游细菌的生物量较高, 而多样性明显偏低, 河道不同采样点的微环境影响浮游细菌 的组成, 河道中浮游细菌的组成与生物量的变化与水环境质量密切相关。 关键词: 河道, 细菌群落, 多样性, 相似性 Bacterial Community Structure in Four Different Rivers of Shanghai in Spring * JIANG Cai-Hong ZHANG Mei-Ling TAO Yan-Jie YU Na CHEN Li-Qiao (College of Life Science, East China Normal University, Shanghai 200062, China) Abstract: In April of 2008 water samples were collected from four different rivers, which were Wuchao gang River, Henggang River, Chaoyang River and Caoyanghuanbang River. During the sampling the physi- cal and chemical parameters were measured. The abundance and the diversity of the bacteria of these four rivers were studied. The results showed that the population level increased in the more severely polluted river while the bacterial diversity decreased; the bacterial community structure was also affected by the dif- ferent ecological conditions of each sampling spot. The bacterial composition and abundance was closely related to the water quality in the river. Keywords: River, Bacterial community, Diversity, Similarity , , , , ,