姜黄素修饰纳米金棒抑制胃癌细胞SGC-7901作用.PDFVIP

安徽农业大学学报, 2016, 43(6): 976-981 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x019 网络出版时间：2016/12/5 10:14:00 [URL] /kcms/detail/34.1162.S1014.038.html 姜黄素修饰的纳米金棒抑制胃癌细胞 SGC-7901 的作用 * 孙冬冬，朱梦瑶，魏雨婷，王海军，汪维云 (安徽农业大学生命科学学院，合肥 230036) 摘 要：恶性肿瘤严重威胁着人类的健康。合成了姜黄素修饰的纳米金棒（Cur@Au NRs），并考察其对胃癌 细胞（SGC-7901）的作用，采用MTT 法测试其对 5 种肿瘤细胞株的体外增殖活性的影响，同时观察其对 SGC-7901 形态学的影响；利用实时 xCELLigence 细胞分析系统 (RTCA)检测不同浓度 Cur@Au NRs 对 SGC-7901 增殖能力及 迁移能力的影响；利用划痕愈合实验及血管形成实验检测 Cur@Au NRs 对细胞的迁移及血管形成的抑制作用；采 用腹腔注射 Cur@Au NRs 后与正常小鼠内脏组织切片的对比，对其进行毒理学研究。研究结果表明，Cur@Au NRs 具有良好的抗肿瘤活性，尤其对 SGC-7901，且其IC50 值明显低于姜黄素，并且能够抑制 SGC-7901 的增殖和迁移。 毒理学研究表明即使在高剂量下也不具有明显的毒性。 关键词：姜黄素；纳米金棒；抗肿瘤；RTCA 中图分类号：Q946.8; R979.1 文献标识码：A 文章编号：1672352X (2016)06097606 The inhibitory effect of gold nanorods through reduction with curcumin on human gastric cancer SGC-7901 cells SUN Dongdong, ZHU Mengyao, WEI Yuting, WANG Haijun, WANG Weiyun (School of Life Science, Anhui Agricultural University, Hefei 230036) Abstract: Malignancies are of the most common illnesses seriously harmful to human health. In this study, gold nanorods were prepared from CTMAB capped Au seeds reduced with curcumin (Cur) to investigate the effect of Cur@Au NRs on human gastric cancer SGC-7901 cells. The MTT method and optical microscope were used to evaluate the effect of Cur@Au NRs on the anti-proliferative activity of tumor cell lines in vitro and the morphology of gastric cancer cells was also observed. The replication of SGC-7901 cells was detected using the real-time cell assay (RTCA) system.