抗菌肽基因的克隆及表达分析.PDFVIP

41 2 Vol.41, No.2 2 0 1 0 3 OCEANOLOGIA ET LIMNOLOGIA SINICA Mar., 2010 (Portunus trituberculatus) Pthyastatin * ( 316004) cDNA Pthyastatin, RT-PCR , Pthyastatin , Pthyastatin 16 , Pthyastatin N Pro/Arg C 6 Cys penaeidins , Pthyastatin penaeidins ; RT-PCR Pthyastatin ; Pthyastatin , 24h , Pthyastatin , , , , Pthyastatin, mRNA Q956 , Penaeidins N , C , 6 Cys, 3 , , , N (Begum et al, 2000) , Cys C , , ; (2) Crustins Crustins , C WAP(whey acidic protein) , , Cys (Cys WAP Penaeidins), WAP 8 Cys / 1, 3- , 4-DSC (Iwanaga et al, 2005) (four-disulphide core); (3) , , C , , (Penaeus stylirostris) (, 2006) PsHct1PsHct2, (Litopenaeus vannamei) PvHct, (Pacifastacus leniusculus) (Lee et al, 2003; Vazquez et al, 2009) (1) astacidin 1 * , 2007BAD43B08 ; ,