抗锰细菌地分离鉴定及其对生猪养殖粪水中锰地去除效果.PDFVIP

() 2015 6 41 3 Journal of Hunan Agricultural University (Natural Sciences), Jun. 2015, 41(3):307–312 DOI:10.13331/ki.jhau.2015.03.016 抗锰细菌的分离鉴定及其对生猪养殖粪水中锰的去除效果 1 1 1 2 1 (1. 411201 2. 529000) 摘 要MnSO4 16S rDNA 0.0050.0100.015 mol/L MnSO4 Mn2+Mn2+ 1) 1 KM01 2) 0.0050.0100.015 mol/L MnSO4 KM01 Mn2+ 28 ℃Mn2+94.8%95.8%89.1% 3) 28 ℃pH 7 1%Mn2+94.8%95.8%89.1% 3) 28 ℃pH 7 1% Mn2+80.5%KM01 关 键 词 中图分类号X592; Q93.331 文献标志码：A 文章编号：1007− 1032(2015)03−0307−06 Isolationidentification of a manganous salt resistant bacterium and manganese removal from pig-breeding fecal wastewater 1 1 1 2 1 Xu Aiqing , Xiang Yanci , Yang Guangxi , Guo Yingxi , Song Zaowen (1. School of Life Science, Hunan University of Science and Technology, Xiangtan, Hunan 411201, China; 2. Jiangmen Entry-Exit Inspection and Quarantine Bureau, Jiangmen,Guangdong 529000, China ) Abstract: To screen manganous-salt resistant bacteria for the microbial treatment of manganese polluted environment. The soil samples were collected from the manganese ore yard and manganese tailing dams in the Xiangtan Manganese Industry Co. Lid.. The manganous salt-resistant bacteria were isolated on manganous sulfate containing solid plate. The morphology characteristics and phylogenic analysis of the 16S rDNA sequence were used for the identification of the strain KM01. The flame atomic absorption method for the determination of the manganese concentration in centrifuged broths containing 0.005 mol/L, 0.01 mol/L and 0.015 mol/L of manganous sulfate was used to calculate the removal rate of manganese. Through an orthogonal experimental design, the liquid ino