猪链球菌2型荚膜缺失株生物学特性与其免疫保护性.PDF

微生物学通报 JAN 20, 2011, 38(1): 85−90 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 研究报告 猪链球菌2 型荚膜缺失株生物学 特性及其免疫保护性 胡丹 王长军 潘秀珍 唐家琪* ( 210002) 摘 要: 对前期构建的猪链球菌2 型中国强致病株05ZYH33 荚膜缺失株Δcps2B 进行相关生物学 特性及免疫保护性研究。通过比较野生株05ZYH33 和荚膜缺失株Δcps2B 生物学特性发现, Δcps2B 株显微形态发生改变, 失去与 2 型荚膜特异抗血清发生凝集反应的能力, 突变株更易被全血清除, 对上皮细胞 HEP-2 的粘附能力增强。动物保护性实验结果表明该荚膜缺失株Δcps2B 具有良好的 免疫保护作用。研究结果提示荚膜在细菌抵抗吞噬和细菌粘附过程中发挥重要作用。 关键词: 猪链球菌2 型, 荚膜多糖, 生物学特性, 免疫保护 Characterization of a nonencapsulated mutant of Streptococcus suis serotype 2 and evaluation of its protective abilities in a mouse model HU Dan WANG Chang-Jun PAN Xiu-Zhen TANG Jia-Qi* (Research Institute for Medicine of Nanjing Command , Nanjing, Jiangsu 210002, China) Abstract: The biological function of a formerly constructed nonencapsuled mutant Δcps2B from 05ZYH33 was characterized, and its protection against infection was evaluated. We found that Δcps2B have reduced ability to form long chain capsule polysaccharide and no longer agglutinated with the specific anti-capsular serum. The Δcps2B could be cleared more easily by the whole blood of human and piglet, while the capacity of adhesion to epithelial cells increased greatly. Animal infection experi- ment demonstrated that the Δcps2B protect mice from lethal challenge with S. suis 2. These observa- tions indicate that the capsule plays an essential role in the pathogenesis and invasiveness of S. suis 2. Keywords: