Human SIRT1 ELISA说明书.doc

na Human Sirtuin 1 (SIRT1) Elisa kit 96 Tests Catalogue Number: E01S0437 Store all reagents at 2-8°C Valid Period: six months For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate FOR RESEARCH USE ONLY! NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING! Table of Contents 1. INTENDED USE 3 2. PRINCIPLE OF THE ASSAY 3 3. Materials 3 4. SPECIMEN COLLECTION AND STORAGE 4 5. MATERIALS AND EQUIPMENT REQUIRED BUT NOT SUPPLIED 5 6. SAMPLE PREPARATION 6 7. REAGENTS PREPARATION 6 8. ASSAY PROCEDURE 6 9. CALCULATION OF RESULTS 7 10. CERTIFICATE OF ANALYSIS 8 11. Safety Notes 9 12. Quality Control 9 13. contact us 10 INTENDED USE 使用意图 This BG SIRT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SIRT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY 试剂盒的原则 SIRT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SIRT1 antibody and an SIRT1-HRP conjugate. The assay sample and buffer are incubated together with SIRT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIRT1 concentration since SIRT1 from samples and SIRT1-HRP conjugate compete for the anti-SIRT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SIRT1 from the sample, fewer sites are left to bind SIRT1-HRP conjugate. A standard curve is plotted relating th