塞来昔布对人乳腺癌SKBR-3细胞生长的影响.PDFVIP

·论 著· 塞来昔布对人乳腺癌SKBR-3 细胞生长的影响 张丽，黄家君*(重庆医科大学药学院药理学教研室，重庆市生物化学与分子药理学重点实验室，重庆 400016) 摘要：目的 探讨选择性环氧化酶-2(COX-2)抑制剂塞来昔布对乳腺癌SKBR-3 细胞生长的影响及机制。方法 用不同浓 度的塞来昔布处理SKBR-3 细胞后，采用CCK-8 法检测塞来昔布对SKBR-3 细胞增殖活性的影响；流式细胞仪检测细胞 周期；酶联免疫吸附试验(ELISA)检测前列腺素 E (PGE )的释放水平；Western Blot 法测定各浓度塞来昔布刺激SKBR-3 2 2 细胞后Caspase-3 被酶解激活情况。结果 塞来昔布对 SKBR-3 细胞的增殖抑制作用呈剂量-时间依赖性；随着塞来昔布 浓度的增加，G0/G1 期细胞阻滞，S 期细胞比例明显减少；塞来昔布明显减少PGE2 的释放水平；Caspase-3 在细胞凋亡早 期被激活，在凋亡晚期则无表达。结论 塞来昔布能有效抑制乳腺癌 SKBR-3 细胞的增殖，诱导其凋亡；其作用机制可 能与COX-2 表达下调、抑制PGE2 水平和促进Caspase-3 的活化有关。 关键词：乳腺癌；SKBR-3；塞来昔布；环氧化酶-2(COX-2) 中图分类号：R965.1 文献标志码：A 文章编号：1007-7693(2012)08-0669-05 Influence of Celecoxib on Brest Cancer Cells SKBR-3 * ZHANG Li, HUANG Jiajun (Department of Pharmacology, School of Pharmaceutical Sciences, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University, Chongqing 400016, China) ABSTRACT: OBJECTIVE To approach the effect of celecoxib, a selective COX-2 inhibitor, on breast cancer cell growth and its mechanism. METHODS CCK-8 assay was adopted to examine the proliferation of SKBR-3 cells treated by different concentrations of celecoxib. Flow cytometry was performed to analyze the cell cycle of SKBR-3. The levels of PGE2 were measured by ELISA. Western Blot was used to detect the activation states of Caspase-3. RESULTS The inhibition of proliferation of SKBR-3 cells in vitro by celecoxib was observed in time- and dose-dependent effects. With the increase of celecoxib concentration, the cell cycle was arrested at G /G , and rate of cells in S-phase was obviously decreased. Levels of 0 1 PGE2 were inhibited by celecoxib. Caspase-3 was activated in the early st