微流体芯片.pptVIP

LOGO Multiple and High-Throughput Droplet Reactions via Combination of Microsampling Technique and Microfluidic Chip Members: 李娜 冯双霞 徐秋燕 刘婷婷 聂怀军 Contents Background Experiment ABSTRACT Discussion ABSTRACT Microdroplets o?er unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based micro?uidics is the di?culty to address droplets individually and achieve high throughput at the same time. Here, we have combined an improved cartridge sampling technique with a micro?uidic chip to perform droplet screenings and aggressive reaction with reagent consumption. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput. Background A large number of reactions can be independently miniaturized into tiny droplets ranging from nanoliter to picoliter sizes without having to increase the chip size and complexity. Microfluidic chips have provided a powerful platform for accomplishing droplet formation and manipulation within one chip of only a few square centimeters in size. Advantages Over the past few years, droplet-based micro?uidics has attracted increasing attention. Background Droplet generation for multiple samples is limited by the “world-to-chip” interface problem and cannot be addressed individually Droplet generation and manipulation functions within one chip can easily interfere with each other and are difficult To balance, despite being separated by a long, winding channel. Drawback Passive droplet formation methods: Innovate This context shows a hybrid device :the cartridge droplet generation technique combined with a microfluidic chip. They improved the cartridge technique with a novel movement scheme: the capillary, instead of the multiwell plate,was moved by a digital and automatic manipulator to aspirate different samples or the covering oil. The hybr