用狂犬病毒核蛋白基因小干扰RNA库抑制狂犬病毒复制与感.PDF

21 3 21 3238-243 2006 5 VIROLOGICA SINICA May 2006 RNA ** 430060 Inhibition of Rabies Virus Replication and Infection by Rabies Virus Nucleoprotein Gene siRNA Cocktail ** WANG Ji-linZHU Jia-hong XU Ge-lin (Wuhan Institute of Biological Products, Wuhan 430060, China) Abstract Small interference RNA mixtures (siRNA Cocktails ) of Rabies virus (RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA (dsRNA) with RNase . These siRNA cocktails were transfecteing into BSR or MNA cell monolayers and the direct immunofluores- cence assay (FA) was used to measure the replication and infection of RV on the cell monolayers. We found that the siRNA Cocktails of N gene can obviously and stably inhibit the replication and infection of RV. A descent of mRNA of N gene was also detected by RT-PCR. Under the same situation, the siRNA Cocktails of P gene or G gene showed no or weak inhibition to the replication and infection of RV. All of these results provide a basis for selecting target gene of RV in RNA itreatment and its further application. Key words Rabies virus (RV) siRNA Cocktail Replication Inhibition RNase RNA N P G RNA siRNA CocktailsiRNA BSR MNA N siRNA Cocktail RV RT-PCR mRNA P G siRNA Cocktail RV RNA RV RVRNA R373.9 A 1003-5125(2006)03-0238-06 RNA (RNAi) 100% RNA [1~3] RNA RNA SARS RV RNase [4~6]