滞育对灰飞虱生理生化特性影响.PDFVIP

植物保护学报Journal of Plant Protection ，2017 ，44 （2 ）：312-317 DOI ：10.13802/j.cnki.zwbhxb.2017.2015171 滞育对灰飞虱生理生化特性的影响 * 宋菁菁 朱文超 林克剑 王桂荣 （中国农业科学院植物保护研究所，植物病虫害生物学国家重点实验室，北京 100193） 摘要：为探明灰飞虱Laodelphax striatellus 滞育过程中生理生化的变化特点，利用蒽酮比色法、甘油 酶法、BCA 法和分光光度法，测定了不同龄期非滞育、滞育灰飞虱若虫体内的总糖、糖原、海藻糖、 甘油、蛋白质和氨基酸含量。结果表明，灰飞虱各龄期滞育若虫体内总糖、糖原、海藻糖、甘油、总蛋 白和总氨基酸的含量与非滞育若虫存在差异。滞育若虫体内糖原和甘油的含量与非滞育若虫无显 著差异，而总糖、海藻糖和氨基酸的含量均显著高于非滞育若虫，3 龄滞育若虫体内海藻糖的含量 为95.5 μg/mg ，是非滞育若虫的1.4 倍；各龄期滞育若虫体内总蛋白含量均极显著高于非滞育若虫， 3 龄滞育若虫体内的总蛋白含量最大，为0.68 g/L ，是非滞育若虫的1.3 倍。研究表明，灰飞虱滞育 个体属于海藻糖积累型，并通过滞育系统调节生理生化习性以增强抗逆性，从而渡过不良环境。 关键词：灰飞虱；滞育；生理；生化；机制 The impact of diapause on physiological and biochemical characteristics of the small brown planthopper, Laodelphax striatellus Song Jingjing Zhu Wenchao Lin Kejian* Wang Guirong （State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Agricultural Academy of Sciences, Beijing 100193, China） Abstract: In order to address the physiological and biochemical responses to diapause of the small brown planthopper, Laodelphax striatellus , the total sugar, glycogen, trehalose, glycerol, total body pro- tein and total amino acid （T- AA ）in different instars of non- diapause nymphs and diapause nymphs were measured with the throne colorimetry, glycerin enzymatic, BCA and spectrophotometric methods. The results showed that total sugar, trehalose and T-AA of diapause nymphs were significantly higher than those of non- diapause nymphs. However, the contents of glycogen and glycerol had no signifi- cance between diapause nymphs and non-diapause nymphs. The trehalose content o