免疫药实验方法-药理实验方法学-研究生.ppt

* anthrax diphtheria tetanus * Cholera 霍乱 Tubercule bacillus结核杆菌 * * One cell type in a mixture is labeled with a specific monoclonal antibody coupled to a very small magnetic bead. The cells are passed over a column which is placed in the magnetic field of a MACS separator. The magnetically labeled cells are retained in the column, the unlabeld cells run through. Then, the column is removed from the magnet and the labeled cells are eluted as the positive fraction. We call this a positive selection. * 2 分光光度法 【基本原理】 又称SRBC的定量溶血测定法，基本原理同溶血空斑法。但本法可定量测定抗体形成细胞所分泌的抗体，较溶血空斑法精确。同时操作比较简便。 * 【操