临床研究ICSI中不同来源精子对卵母细胞受精、胚胎质量及发育潜能的详解.pdfVIP

J South Med Univ, 2014, 34(6): 857-861 doi 10.3969/j.issn. 1673-4254.2014.06.21 ·857 · 临床研究 IICSICSI 中不同来源精子对卵母细胞受精中不同来源精子对卵母细胞受精、、胚胎质量及发育潜能的胚胎质量及发育潜能的 影影响响 谢 多，邱卓琳，罗 琛，褚庆军，全 松 南方医科大学南方医院生殖医学中心，广东 广州 510515 摘要：目的研究卵胞浆内单精子注射技术（ICSI）中不同来源精子对卵母细胞受精、胚胎质量及发育潜能的影响。方法 回顾性 分析我中心因单纯男性因素行ICSI 助孕治疗的197例患者的临床资料，按不同精子来源分为射精组（n= 102）、PESA 组（n=68）、 TESA 组（n=27）3 组，其中将射精组又分为严重少弱畸精子症组（n=67）和隐匿精子症组（n=35）。比较4 组间受精率、优质胚胎 率、新鲜胚胎移植周期的着床率及妊娠率，以及低质胚胎继续培养的优质囊胚形成率。结果 少弱畸精子射精组、隐匿精子射精 组、PESA 组、TESA 组的正常受精率分别为64.8%、62.1%、75.6 %、61.6% ，其中PESA 组明显高于其它3 组（P0.05），4 组的优质 胚胎率、新鲜胚胎移植周期的着床率及妊娠率无明显差异（P0.05）。4 组间低质胚胎继续培养的优质囊胚形成率也无显著差异 （P0.05）。结论PESA 来源的精子ICSI 中正常受精率较高，但在ICSI 中不同来源精子对胚胎质量及发育潜能无显著影响。 关键词：卵胞浆内单精子注射技术；精子来源；胚胎质量；胚胎发育潜能 Effect of spermatozoa from different sources on normal fertilization of oocytes and embryo quality and development in intracytoplasmic sperm injection cycles XIE Duo, QIU Zhuolin, LUO Chen, CHU Qingjun, QUAN Song Center of Reproductive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To evaluate the impact of spermatozoa from different sources on normal fertilization of oocytes, embryo quality and embryo developmental potential in intracytoplasmic sperm injection (ICSI) cycles. Methods A retrospective analysis was conducted among 197 patients undergoing ICSI cycles in our center. The patients were classified into 3 groups according to the sources of semen, namely ejaculated spermatozoa group (n=102), percutaneous epididymal sperm aspiration (PESA) group (n=68), and testicular sperm aspiration (TESA) group (n=27). The ejaculated spermatozoa group was further classified into oligoasthenoteratozoospermia (n=67) and cryptozoospermia (n=35) subgroups. The normal fertilization, high-quality embryo, implantation and clinical pregnancy rates were compared among