CEAmRNA论文.docVIP

　　CEAmRNA论文 【关键词】 癌胚抗原 Different expression and clinical application of CEAmRNACEAp system in benign and malignant ascites 【Abstract】 AIM: To understand the different expression of carcinoembryonic antigen mRNA (CEAmRNA)carcinoembryonic antigen protein (CEAp) system in benign and malignant ascites and to assess the value of CEAmRNACEAp for the diagnosis and differential diagnosis of benign and malignant ascites. METHODS: Niythree patients ined, among 60 cases alignant ascites and 33 cases RNA and CEAp detection. CEAmRNA erspolymerase chain reaction (RTNPPCR) and CEAp agic antibody immunoenzymometric assay (MAIA). RESULTS: The positive rates of CEAmRNA and CEAp alignant ascites group and 1 (3.0%) and 3 (9.1%) in the benign ascites group.The positive rates of both CEAmRNA and CEAp in the ascites of patients alignant ascites alignant ascites, the specificity, sensitivity, the positive likelihood ratio, the negative likelihood ratio, the positive predictive value, the negative predictive value and the accuracy of CEAmRNA and of CEAp RNA and CEAp are useful indexes for the diagnosis and differential diagnosis of malignant ascites. The diagnostic efficiency of CEAmRNA is higher than that of CEAp. 【Keybryonic antigen; RNA, messenger; carcinoembryonic antigen protein; diagnosis, differential; ascites, malignant 【摘要】 目的： 了解癌胚抗原信使核糖核酸(CEAmRNA)癌胚抗原蛋白(CEAp)系统在良、恶性腹水中表达的差别，评估检测CEAmRNACEAp系统指标诊断与鉴别诊断良、恶性腹水的应用价值. 方法： 恶性腹水组60例，良性腹水组33例. 采集腹水为检测标本，应用反转录套式聚合酶链反应(RTNPPCR)技术检测CEAmRNA，磁性抗体分离酶免疫测定(MAIA)技术检测CEAp. 结果： 良、恶性组腹水中CEAmRNA和CEAp的阳性率分别为3.0%(1/33)和9.1%(3/33)，81.7%(49/60)和56.7%(34/60)，良、恶性腹水间均具有显著的统计学差异(P 0.01). 用CEAmRNA和CEAp指标诊断与鉴别诊断恶性腹水的特异度、敏感度、阳性似然比、阴性似然比、阳性预测值、阴性预测值、准确度分别为0.970和0.909, 0.817和0.567, 27.233和6.231, 0.189和0.476, 0.980和0.919, 0.744和0.544, 0.871和0.699. 结论： 检测腹水中CEAmRNA和CEAp对良、恶性腹水的诊断与鉴别诊断均具有较高的应用价值，其中以CEAmRNA指标更为重要. 【关键词】 癌胚抗原；RNA.freelbryonic antigen mRNA, CEAmRNA)癌胚抗原蛋白(carcinoembryonic antigen protein, CEAp)系统在良、恶性腹水中的表达水平具有非常显著差别. 我们应用CEAmRNACEAp系统指标诊断与鉴别诊断良、