重组人粒细胞巨噬细胞集落刺激因子发酵工艺和液相色谱法对其复性并同时纯化.pdf

1．66x107IU／mg，质量回收率为58．8％，纯度可达到96．2％。 5．SEC对rhGM．CSF复性并同时纯化 应用SEC法对rhGM．CSF进行了复性并部分纯化，研究了流动相中脲浓度、 中有脲存在并用脲梯度是一种更有效的SEC复性rhGM-CSF的方法。 关键词：重组人粒细胞．巨噬细胞集落刺激因子，基因工程，大肠杆菌，摇瓶发 酵，复性，分离纯化，疏水色谱，离子交换色谱，排阻色谱，简易型色谱柱与 ABSTRACT ofdenatured Refolding proteknsbyliquid a chromatography(LC)isnewlydevelopping denaturedcanberenaturedwihsimultaneous technique．The withLC． protein purification Itcan the renatu觚on ithas ofthe been much improve efficiencytargetproteins，s0 paid attentioninrecent this fermentationconditionsand years．Indissertation，the enlarged craRworkfor rhGM·CSF studied．The E．coli，were and expressedby llig}lly expressed rhGM-CSFcanbeobtainedunderthe output fermentationcondition． highly optimal In renatumfionwithsimultaneousofrhGM-CSFwas addition,the purification investigated with perfommce interaction high hydrophobicchromatography(HPHIC)，highperformance iron sizeexclusion exchangechromatography(HPmC)and Thedissertationincludesfivesections： 1．Review Significance isintroduced a review ofproteinrefolding briefly,andcomprehensive on is recent recentlydevelopedtechniquesofproteinrofoldingprovided．Commem