鸡禽流感9型（AIV-9）酶联免疫分析（ELISA）.docVIP

鸡禽流感9型（AIV-9）酶联免疫ELISA） 试剂盒使用说明书-20℃保存，但应避免反复冻融操作步骤μl。然后在待测样品孔μl，然后再加待测样品10μl。加样将样品加于酶标板孔底部，尽量不触及孔壁，轻轻混匀，分钟甩干μl，空白孔除外。 μl，再加入显色剂B 50μl，轻轻震荡混匀，37℃避光显色15分钟 终止：每孔加终止液50μl，终止反应。450nm波长依序测量各孔的度（OD值）≥1.00; 阴性对照平均值≤0.10 临界值（CUT OFF）计算：临界值=阴性对照孔平均值+0.15 阴性判定：样品OD值 临界值（CUT OFF）者为禽流感9型（AIV-9）阴性 阳性判定：样品OD值≥ 临界值（CUT OFF）者为禽流感9型（AIV-9）阳性 注意事项 1．操作严格按照说明书进行，本试剂不同批号组分不得混用。 2．试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。 3．浓洗涤液会有析出，稀释时可在水浴中加温助溶1．；2-8。 Chicken AIV-9 FOR RESEARCH USE ONLY Drug Names Generic Name：Chicken AIV-9 ELISA Kit. Purpose This kit allows for the determination of AIV-9 concentrations in Chicken serum, and other biological fluids. Principle of the assayThe kit assay AIV-9 level in the sample，use Purified AIV-9 anti to coat microtiter plate wells, make solid-phase antibody, then add AIV-9 to wells, Combined With AIV-9, after washing and removing non-combinative antibody and other components ,then Combined AIV-9 antibody which with HRP labeled become antibody - antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according to this to judge AIV-9 exist in the sample or not. Materials provided with the kit Materials provided with the kit 48determinations 96 determinations Storage User manual 1 1 Closure plate membrane 2 2 Sealed bags 1 1 Microelisa stripplate 1 1 2-8℃ Negative control 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃ Positive control 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃ HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃ Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃ Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃ Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃ Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃ wash solution （20ml×20 fold） ×1bottle （20ml×