绿色棉新彩棉7号体细胞胚胎发生及其植株再生.pdfVIP

花 学 报 Cotton Science 20 13 25 渊2 冤院110~ 114 色棉新彩棉7 号体细胞胚胎发生及其植株再生 * * ** 朱华国 王红娟 李鹏飞 张新宇 李艳军 孙 杰 石河子大学农学院/ 新疆生产建设兵团绿洲生态农业重点实验室袁新疆石河子832003 冤 摘要院以绿色棉新彩棉7 号的子叶尧下胚轴为外植体袁MSB(MS 培养基附加B5 维生素)基本培养基附加不同激 素组合袁诱导愈伤组织及调控分化袁通过体细胞胚胎发生方式获得再生植株遥结果表明院0.1 mg 窑L-1 KT(Kinetin, 激动素)+ 0.1 mg 窑L-1 2,4-D(2, 4-dichlorophenoxyacetic, 2,4- 二氯苯氧乙酸) 为诱导愈伤组织的最适植物激素组 合袁不同外植体处理出愈率均达到 100 袁但下胚轴纵 面背向培养基放置培养更有利于诱导愈伤组织形成曰 分化调控阶段的最佳植物激素组合为0.15 mg 窑L-1 KT + 0.3 mg 窑L-1 IBA(Indole-3-butyric acid, 吲哚丁酸) 袁胚性 愈伤分化率可达23.33% 曰MSB 中去除NH NO 同时KNO 加倍袁附加0.5 g 窑L-1 Asn(Asparagine, 天冬酰胺)和1 4 3 3 g 窑L-1 Gln(Glutamine,谷氨酰胺) 袁胚性愈伤可进一步分化获得体细胞胚袁将成熟的子叶胚接种于1/2MS 获得完 整的再生植株遥 本研究通过体细胞胚发生途径获得了新彩棉7 号的再生植株袁为天然彩色棉基因工程研究奠 定了一定基础遥 关键词院绿色棉曰体细胞胚胎发生曰植株再生 中图分类号院S562.01 文献标志码院A 文章编号院1002-7807(2013)02-0110-05 * * ** ZHU Hua-guo , WANG Hong-juan , LI Peng-fei, ZHANG Xin-yu, LI Yan-jun, SUN Jie ( 832003 ) Using hypocotyls and cotyledon of Xincaimian No.7 (Green fiber cotton cultivar) as explants, MSB (MS salts and B5 vitamins) medium supplemented with different phytohormone combinations as callus induction media, and by embryogenic callus differentiation, regeneration experiment was carried out. The results indicated that the calli were easily induced in medium supplemented with 0.1 mg 窑L-1 2 4-D and 0.1 mg 窑L-1 KT, the frequency of callus induction was 100%. Split hypocotyl segments reversely cultured on medium was the best mode for callus induction. 0.3 mg 窑L-1 IBA and 0.15 mg 窑L-1 KT was the best phytohormone combination