越橘葡萄座腔菌枝枯病的病原菌鉴定.pdfVIP

园 艺 学 报 2013 ，40 （2 ）：231 –236 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 越橘葡萄座腔菌枝枯病的病原菌鉴定 * 徐成楠，周宗山 ，迟福梅，吴玉星，冀志蕊，张红军 （中国农业科学院果树研究所，辽宁兴城 125199 ） 摘 要：通过形态学观察、诱导产孢、致病力测定和分子生物学研究，对引起辽宁地区越橘枝枯病 的病原菌进行鉴定，确定其为葡萄座腔菌（Botryosphaeria dothidea ）。病原菌在越橘植株上接种后表现枝 条枯萎、木质部变色等症状，与田间自然发病植株一致；在 PDA 培养基上菌落初期为白色，后转为灰黑 色，分生孢子纺锤形或梭形，无色，单胞，内含多个油滴；水琼脂 + 麦芽提取物、苹果枝条和苹果幼果 3 种病原菌培养方法均可诱导产孢，苹果幼果是诱导病原菌产孢的最佳基质。供试菌株 LB1 的rDNA-ITS 和 β-tubulin 基因序列与 GenBank 中已有的Botryosphaeria dothidea 序列相似性分别达到 99%与 100%。 关键词：越橘；枝枯病；葡萄座腔菌；Botryosphaeria dothidea ；诱导产孢 中图分类号：S 663.2 文献标志码：A 文章编号：0513-353X （2013 ）02-0231-06 Research on the Pathogen Causing Botryosphaeria Stem Blight on Blueberry * XU Cheng-nan ，ZHOU Zong-shan ，CHI Fu-mei，WU Yu-xing ，JI Zhi-rui ，and ZHANG Hong-jun （Research Institute of Pomology ，Chinese Academy of Agricultural Sciences，Xingcheng ，Liaoning 125199，China） Abstract ：The pathogen causing stem blight on blueberry in Liaoning，China was identified as Botryosphaeria dothidea by morphological characteristics ，sporulation features，pathogenicity test ，and the molecular identification. The stems showed symptoms of dieback ，discoloration in trunks and death after inoculating with the pathogen，which were consistent with the symptoms of the natural infected plants. All isolates showed the colonies white at the early stage ，then became gray to black ，conidia were hyaline， aseptate，fusiform with several oil globules，young apple fruit is the suitable medium for sporulation. Identity of isolate LB1 was confirmed by analysis of the rDNA-ITS and β-tubulin sequences with primers ITS1 –ITS4 and Bt2a –