魔芋组培快繁的影响因素.pdfVIP

中国蔬菜 2012（8）：75-79 CHINA VEGETABLES 魔芋组培快繁的影响因素 * 胡 悦 冉兴宇 张兴国 苏承刚 （西南大学园艺园林学院，南方山地园艺学教育部重点实验室，重庆 400715） 摘 要： 以万源花魔芋顶芽、球茎为外植体，比较不同激素浓度配比对外植体培养的影响，并进一步 诱导试管微型芋。结果表明：顶芽是魔芋组培快繁的最佳外植体，腋芽产生频率高、速度快，较球茎分化 不定芽的时间提早 60 d 左右；腋芽分化适宜培养基是 MS + BA 2.4 mg·L-1 + NAA 0.1 mg·L-1 ，腋芽分化率 90%；在增殖培养基中腋芽增殖系数达 3.58；腋芽在 MS + BA 0.5 mg·L-1 + NAA 0.01 mg·L-1 + 蔗糖 50 g·L-1 培养基中诱导形成微型芋，培养 60 d 的诱导率达 90.9%。 关键词：魔芋；腋芽；分化；诱导；微型芋 中图分类号：S632.9 文献标识码：A 文章编号： 1000-6346（2012）08-0075-05 Factors Influencing Rapid Propagation of Konjak Tissue Culture * HU Yue，RAN Xing-yu，ZHANG Xing-guo，SU Cheng-gang （College of Horticulture and Landscape Architecture，Southwest University ，Key Laboratory of Horticulture Science for Southern Mountainous Regions ，Ministry of Education ，Chongqing 400715，China ） Abstract ：Apical buds and corm of‘Wanyuanhuamoyu’（Amorphophallus spp.）were taken as explants，with various concentrations of BA，KT，ZT and NAA were studied．Then the induction of micro corms were investigated from axillary buds．The results showed that the apical bud was the best explant in rapid propagation of konjak tissue culture，the highest induction rate of 90% of axillary buds was obtained from apical buds on the medium（MS + BA 2.4 mg·L-1 +NAA 0.1 mg·L-1 ）．And the differentiation time of axillary buds form apical buds was 60 days earlier than that from corm．Micro -1 -1 corms in vitro were generated on the medium（MS + BA 0.5 mg·L + NAA 0.01 mg·L + sucrose 50 g·L-1 ）．The callus induction rate was 90.