物料预处理对酶法提取灵芝多糖的影响大学学位论文.docVIP

毕业设计(论文) 课 题 名 称 物料预处理对酶法提取灵芝多糖的影响 学 生 姓 名 学 号 系、年级专业 生物与化学工程系2011级生物工程 指 导 教 师 职 称 讲 师 2015 年 5 月 20 日  物料预处理对酶法提取灵芝多糖的影响 摘 要 目的:经过对灵芝中灵芝多糖的工艺提取的研究，找出最佳的物料预处理方法处理灵芝，然后进行提取，让灵芝多糖的提取率尽可能的达到最大值。方法:本实验主要采用酶法提取灵芝多糖的方法，以灵芝多糖的提取率为目的，进行各项单因素实验探究，考察物料烘烤温度、物料粒度和紫外线的照射时间等物料预处理的条件对灵芝多糖提取的影响。最后紫外分光光度计测定灵芝多糖的质量，并得出最佳的实验条件。结果:经由实验结果整理分析可以得知，单因素的影响大小为 B（A烘烤温度，B物料粒度，C紫外线照射时间），其最佳组合为B2C2A1，粉细度为80目，紫外线照射时间为35 min，烘烤温度为75℃，灵芝多糖提取率最高，能够达到1.55%。结论：通过对物料的预处理可以发现，较之未经物料预处理的提取，灵芝多糖的提取率显著提高。 关键词: 预处理；灵芝多糖；酶法提取；紫外-可见分光光度法 The material pretreatment effects on enzymatic extraction of GLP (2011 Biological Engineering , Department of Biology and Chemistry Engineering, Shaoyang Abstract Objective: To find out the best material pretreatment extraction technology makes the GLP extraction yield as high as possible by studying on the extraction and purification of Ganoderma lucidum polysaccharides(GLP) from Ganoderma lucidum.Methods: To investigate the baking temperature, material size and UV processing time of material pre-treatment conditions on extraction of GLP influence based on single factor experiment, using enzymatic extraction method and taking GLP’s yield as the index. To optimize the optimum conditions of extracting GLP from Ganoderma lucidum powder by the orthogonal test, which apply ultraviolet and visible spectrophotometry (UV-vis) to determine the content of GLP. Results: The results of orthogonal test show that the main factors which influence GLP’s yield in enzymatict extraction with the material pre processing are as follows: B or C A(A is the baking temperaturet, B is the material size, C is UV processing time), and the best parameter combination is B2C2A1.That is to say, The optimum conditions in ultrasonic enzymatict extraction with th