汞胁迫对水葫芦光合特性及生理生化特征影响.docVIP

汞胁迫对水葫芦光合特性及生理生化特征影响 　　摘 要：当前我国水体汞污染日益严重，由于其易迁移性和毒性，严重威胁着生态环境和人类的健康，寻找对汞胁迫具有一定抗性的水生植物已经刻不容缓。试验采用溶液培养法研究不同质量浓度的HgCl2（0，2.5，5.0，10.0，20.0，40.0 mgL-1）对水葫芦幼苗生长和发育的影响。结果表明，水葫芦鲜质量增长量、叶绿素含量、Pn值和SOD活性在低质量浓度的HgCl2溶液中随着汞质量浓度的升高而增加，在质量浓度为5 mgL-1时达到最高点；但随着HgCl2质量浓度进一步增大，这些指标值反而持续下降。Pro和SP含量在HgCl2质量浓度为10 mgL-1时达到最高点，但当HgCl2质量浓度达到20 mgL-1时较对照显著下降；而Gs，Ci，Tr值表现出先下降后上升的趋势，但各处理间差异不显著，其中，Ci，Tr值在HgCl2质量浓度为5 mgL-1时达到最低值，Gs在10 mgL-1时达到最低值；MDA含量和POD活性则随着HgCl2质量浓度的升高而上升。水葫芦对10 mgL-1 以下的HgCl2胁迫具有较好的适应性，且可促进水葫芦的生长；但高质量浓度HgCl2对水葫芦的生长则有抑制作用。研究结果可为水葫芦对Hg2+胁迫的适应机制提供一定理论依据 关键词：水葫芦；汞胁迫；叶绿素含量；光合特性；生理生化特征 中图分类号：S555+.5 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2017.05.002 Abstract： The water mercury pollution has been seriously polluted in recently years in our country， because of its easy mobility and toxicity serious threat to the ecological environment and human health seriously， therefore to search for the hydrophyte with some resistance to mercury stress was extremely urgent. The aim of the study was to investigate the influence that HgCl2 under various concentrations（0， 2.5， 5.0 10.0， 20.0， 40 mgL-1） on the growth and development of Eichhornia crassipes seedlings with hydroponics. The results indicated the FW increment， chlorophyll content， Pn value， SOD activities of Eichhornia crassipes increased first and then decreased with the increasing of HgCl2 stress. The highest value at 5 mg?L-1， but as HgCl2 concentration increased further， these indicators continued to decline inversely. Pro and SP reached to the highest point at the 10 mgL-1 concentration， and they continued to decline at the concentration more than 20 mgL-1 significantly. While Gs， Ci， Tr showed the opposite tendency compared with Pn. But it was not significant between difference HgCl2 stress. Ci， Tr reached to the highest point at the 5 mgL-1 concentration， but Gs at 10 mgL-1. The MAD content and POD activity significantly rose with the increasing of HgCl2 stress. The conclusion was that it was beneficial to Eichhornia cra