多粮浓香型白酒厂内细菌多样性及分布的研究.pdfVIP

※生物工程 食品科学 2010, Vol. 31, No. 13 209 多粮浓香型白酒厂内细菌多样性及分布的研究 周瑞平，陈云宗，唐代云 (四川省宜宾市叙府酒业有限公司技术中心，四川宜宾 644000) 摘 要：采用改良的N A 培养基分离、去除冗余，从多粮浓香型酒厂内的空气、曲药、糟醅、窖泥中分离到 82 株细菌，其中优势细菌24 株，占分离所得细菌总数的29.2 7% 。对这24 株优势细菌的16S rDNA 序列分析表明， 其中13 株为Bacillus 属，9 株分属于Ly s inibacillus 属、Stap hy lococcus 属、R ummeliibacillus 属，Brevibacillus 属、 B rachy bacterium 属，还有2 株菌与数据库中模式菌株相似性低于97% ，很可能代表着新类群。入窖糟醅中细菌种 类及数量最多，曲药中产芽孢细菌最多，可产淀粉酶的细菌在生产环境中分布最广，表明细菌种类及数量的分布 与白酒生产以及酒体的风格形成可能有着密切的联系。 关键词：多粮浓香型；细菌；分离；16 S r D N A ；分析 Diversity and Distribution of Bacteria in Luzhou-flavor Wine Factory ZHOU Ru i-ping ，CHEN Yun-zong ，TANG Dai-yun (Technical Center, Xufu Liquor Industry Co. Ltd., Yibin 644000, China) Abstract ：Totally 82 bacterial strains were separated from air, koj i, grain stuff and pit mud in Luzhou-flavor wine factory using improved NA medium, of which 24 were the dominant strains, accounting for 29.27% of the total number. According to the 16S DNA sequence analysis, the 24 strains comprised 13 belonging to the genus Bacillus, 9 belonging to the genera Lysinibacillus, Stap hy lococcus, Rummeliibacillus, Brevibacillus or Brachy bacterium and 2 presumed to be new species belonging to the genera Bacillus or Brevibacillus due to less than 97% similarity in 16S rDNA gene sequence compared with type strains from database. Meanwhile, most species and population of bacteria were observed in grain stuffs prepared for fermentation and endospore- bearing bacteria were observed in koj i. However, amylase-producing bacteria were distributed in manufacturing environment. These findings suggest that the distribution of bacterial species and their number