棉花重要抗黄萎病相关基因的表达特性分析及GhPR10功能初步研究.pdf

烟草和拟南芥的表型，转基因植株生长延缓，转基因拟南芬叶片倾向于横向生长而呈近圆形，顶 端优势减弱，侧生茎数量显著增多，表明GhPRIO参与调控植物的生长发育。 关键词： 棉花抗黄萎病相关基因，表达特性，RACE与TAIL-PCR，GhPRIO，转基因 n Abstract is inboththe and Verticilliumwiltofcottona diseasewhichcausesreduction major quality ofcottonandresuRsinsubstantialeconomic withcombinationof quantity losses．Applied andtraditional canscreencottonresistance-related new biotechnology breeding．we genes,cultivate breed whichistheeasiesttocontroldisease． directionally way We RT-PCRtotest8 acidandabsisicacidinfectedcotton u$e pathogentoxin,salicylic seedlings． 22eDNA which subtractive analysis sequencesacquiredbysuppressionhybridization，homologcloning so be Callbe and011．19outofthese22 can inducedVerticillium induced genes by dahliae，17 by Call Aspergillusnigervan脚and response tOinfectionofmostof wiltofenttonresistance-relatedGhVElcallbe pathogen，Verticillium gene inducedVdah／iae andGhVDRGllatetransient by specifically．GhPRIO,GhGGH expressiongenes detectedin be 30minto a∞ whichcanbe 10minutes．14can induced 90min．15 genes during genes after6 Verticilliumwiltofcottonresistance-related and co-expressedhours，comprisingGhVE2，GhEH GhVEI．In