空肠弯曲菌cdtA、cdtB、cdtC基因构建与表达.pdf

目 录 0000000 中文摘要……0 0………………………………………………………1 英文摘要……………………………………………………………………s 研究论文 空肠弯曲菌cdtA、cdtB、cdtC基因构建与表达 引言……………………······………··……·……···………．．．…．．．．．．9 第一部分 空肠弯曲菌候选疫苗基因的T—A克隆 —1上．—j一 日IJ吾””””“””“……”一一“一”“．．…．．．．．．…·…························12 材料与方法……………………………………………………………13 结果……………………………………………………………………19 附图……………………………………………………………………20 附表……………………………………………………………………28 讨论······…··………·················…····………····…······．·．．．．．．…．30 小结……·······…··…·········…………····…·············．．…．．．…．．．．．32 参考文献………………………………………………………………32 第二部分 空肠弯曲菌cdtA、cdtB、cdtC基因的克隆、序列分析 及B细胞表位测 —jL．．1一 刖舌“”““”““”””“””……““．．…．．．．．．······………···…··········35 材料与方法……………………………………………………………35 结果……………………………………………………………………37 附图……………………………………………………………………41 讨论···············………···………·························……．．．．．．．．．．．46 小结··········……………··…··············…···…···…··．．．．．．…．．．．．．．．47 参考文献………………………………………………………………47 第三部分空肠弯曲菌cdtA及其高抗原肽段的原核重组质粒的构建及表达 ．^L—jL· 月IJ舌”一“…”“”“一”””“一“一………．．一”·················……··…··50 材料与方法……………………………………………………………51 结果……………………………………………………………………53 附图……………………………………………………………………54 讨论…·····……·······…······················……·········．·．·．．．．．．．．．．…56 小结············…······………···…·························．·…．．．．．．．．．．．．56 参考文献………………………………………………………………57 前言………………………………………………………………···…58日IJ舌…“………”””””””””””””””………一”““”“………““58 材料与方法……………………………………………………………59 OOO O OOOOOO 结果…”””…”””””””一““一””““······…·········I O O OOOOO 61 O OOOOOOOO 附图…………0O O………………………………………………62 讨论…··………········…····…··························……·····…·…··63 ／j、结···················…·…·…········一················一··············