安定对大鼠局灶性脑梗死半影区神经细胞死亡的保护作用论文.docVIP

　　安定对大鼠局灶性脑梗死半影区神经细胞死亡的保护作用论文 .freelg kg-1 8h-1 ），直到处死动物. 结果 TUNEL阳性细胞位于坏死中心与正常皮层之间的半影区.TUNEL阳性细胞的均数随着梗死后时间的延长逐渐增多，24h达到高峰.在24h时间点.freel;apoptosis;necrosis;cerebral ischemia;cerebral infarction Abstract:AIM To study the protective effects of diazepam against nervous cell death after photochemically induced cere-bral ischemia.METHODS Focal cerebral ischemia induced by photothrombosis in adult rats odel.Apop-tosis inal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling（TUNEL）.The areas of necrotic cores and the numbers of apoptotic cells （10mg kg-1 8h-1 ）als al cortex.The mean num-ber of TUENL-positive cells e increased from3，6to12h and peaked at24h.The mean maximum area of the necrotic cores and mean number of TUNEL-positive cells at24h -treated experi-mental group than in the saline-treated control group.CONCLUSION Diazepam can significantly reduce both necrosis and apoptosis folloia induced by photothrombosis in adult rats.These findings provide a theo-retical in ischemic cerebrovascular diseases. 0 引言 近年来有研究显示，安定具有保护脑部缺血性神经元，防止其退行性变的作用［1，2］ .我们采用大鼠光化学局灶性脑梗死模型，观察安定对大鼠脑梗死后半影区神经细胞凋亡的影响，旨在探索治疗脑梗死的新途径与方法. 1 材料和方法 1.1 材料 SD雄性大鼠48只，体质量200～250g，由第四军医大学实验动物中心提供.其中36只大鼠按术后存活3，6，12，24，48和72h随机分为6组，每个时间点6只;另外12只大鼠被随机分为安定治疗组和对照组，每组6只. 1.2 方法 1.2.1 大鼠光化学脑梗死模型 动物均采用10g L-1 戊巴比妥钠50mg kg-1 ，腹腔注射麻醉，俯卧位固定于脑立体定向仪上（Narishiga，日本）.常规皮肤消毒，正中矢状切开头皮，细心分离部分颞肌以暴露顶叶，光导纤维（孔径3mm）置于前囟后3mm、矢状缝左旁开3mm之交点处，光纤与颅骨表面距离为2mm.其下对应于顶叶感觉皮层（parietal sensory cortex）.光照前1min内自尾静脉注射玫瑰红B（10g L-1 ，生理盐水配制），剂量25mg kg-1 .光照强度1ed Univ，2001;21（2）:102-104. ［2］Do-induced neuroprotection:Dissociating the effects of hypothermia folloia ［J］.Brain Res，1999;829（1-2）:1-6. ［3］Sch RD，McDonough KJ，Chase PJ，Chad neuroprotection by benzo-diazepine full versus partial agonists after transient cerebral is-chemia in the gerbil ［J］.J Cereb Blood Floent of focal cerebral is-chemia.Part II:Mechanisms of damage and treatment ［J］.J Neurosurg，1992;77:337-354. ［5］Kharlamov A，Uz T，Joo JY，Mane