红树内生细菌CⅢ-1菌株鉴定及其胞外抗菌蛋白性质.PDF

微生物学通报 FEB 20, 2010, 37(2): 222−227 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 红树内生细菌 CⅢ-1 菌株鉴定及其胞外 抗菌蛋白性质 * 张兴锋 柳凤 何红 陈振明 胡汉桥 ( 524088) 摘 要: 用对峙生长法从 422 株红树内生细菌中筛选到 1 株对青枯雷尔氏菌(Ralstonia solanacea- rum) 、金黄色葡萄球菌(Staphylococcus aureus) 、溶藻弧菌( Vibrio alginolyticus) 、辣椒疫霉菌 (Phytophthora capsici) 、香蕉枯萎病菌(Fusarium oxysporum f. sp. cubense)等动植物病原细菌和真菌均 具有较强的拮抗作用的海洋细菌CⅢ-1 菌株, 经形态和生理生化特性及16S rRNA序列分析, 鉴定该 菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens) 。用33%硫酸铵从菌株的培养滤液中盐析获得对 供试病原菌均具有较强拮抗作用的抗菌活性蛋白, 性质测定发现, 该抗菌活性蛋白对热不稳定, 60°C 和100°C 下处理10 min, 其抗菌活性分别下降了62.5%和完全丧失, 在pH 5.0− 10.0 范围内均具 有抑菌作用, 以pH 7.0 时抗菌活性最强。 关键词: 红树, 内生细菌, 鉴定, 抗菌蛋白, 分离 Identification of Endophytic Bacterium CIII-1 Strain from Mangrove and Isolation of Its Antimicrobial Proteins * ZHANG Xing-Feng LIU Feng HE Hong CHEN Zhen-Ming HU Han-Qiao (College of Agriculture, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China) Abstract: The CⅢ-1 strain, a marine bacterium screened out from 422 endophytic bacteria isolated from mangroves by the method of plate test, strongly inhibited the growth of Ralstonia solanacearum, Staphylo- coccus aureus, Vibrio alginolyticus, Phytophthora capsici, Fusarium oxysporum f. sp. cubense, etc.. It was identified as Bacillus amyloliquefaciens based on the characters of morphology, physiology and biochemis- try an