纳米铁对大肠杆菌毒性作用机理研究-生态毒理学报.docVIP

零价纳米铁对大肠杆菌的毒性研究 王学，李勇超，李铁龙，金朝晖 1.南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室,天津300071 2.天津市城市生态环境修复与污染防治重点实验室，天津300071 N-乙酰半胱氨酸(NAC)后细胞生长的变化。零价纳米铁可导致细胞培养液上清中LDH活性显著升高，细胞内SOD活性显著下降、MDA含量显著升高，且含量变化与零价纳米铁浓度存在剂量-效应关系。加入抗氧化剂NAC后，加NAC的实验组大于没加NAC的实验组。实验结果表明零价纳米铁的毒性机制为氧化损伤。 关键词：零价纳米铁；大肠杆菌；生长曲线；酶活性；氧化损伤 文章编号：中图分类号：X523 文献标识码：A Toxicity Effects of ano-Fe0 on Escherichia coli Wang Xue, Li Yongchao, Li Tielong*, Jin Zhaohui 1. Key Laboratory of Pollution Processes and Environmental Criteria of Ministry of Education, College of Environmental Science and Engineering, Nankai University,Tianjin 300071 2.Tianjin Key Laboratory of Environmental Remediation and Pollution Control,Tianjin 300071 Abstract: The cytotoxicity effects and mechanism of the nano-Fe0 on Escherichia coli were studied. In this paper, morphological changes of E. coli due to the presence of nano-Fe0 were observed by transmission electron microscopy (TEM). E.coli cells were then exposed to 0, 112, 560, 1120 mg/L of nano-Fe0 particles, respectively. Experiments were carried out to examine the inhibition effect of nano-Fe0 on E.coli growth.The activities of lactate dehydrogenase (LDH), cellular superoxide dismutase (SOD), malondialdehyde (MDA) were also determined after exposure to nano-Fe0 for 24h. Moreover, the influence of addition of N-Acetylcysteine (NAC) on the viability of cells was compared. TEM images of E.coli cells treated with nano-Fe0 exhibited significant disruption of the cell membranes. Experimental results of growth curves showed that nano-Fe0 nanoparticles could inhibit the growth of cells in liquid LB medium; nano-Fe0 shortened the log phase and prolonged the stable phase of E.coli, and the higher of the concentration of nano-Fe0 particles, the longer of the stabilization phase of E.coli. The activities of LDH and the levels of MDA significantly increased, respectively, the activities of SOD were significantly decreased;?there was a linear relationship between the concentration of nano-Fe0 and MDA levels.?In n