西葫芦种质资源遗传多样性的 RAPD 分析.pdfVIP

中国蔬菜 2010（16）：26-31 CHINA VEGETABLES 西葫芦种质资源遗传多样性的 RAPD 分析 1 2* 陈凤真 何启伟 1 2 （菏泽学院园林工程系，山东菏泽 274000； 山东省农业科学院蔬菜研究所，山东济南 250100） 摘 要：利用 RAPD 技术对国内外 47 份西葫芦种质材料进行遗传多样性及亲缘关系分析。从 520 条 随机引物中筛选出 30 条能产生稳定多态性的引物用于 RAPD 分析，共扩增出 367 条带，其中 302 条表现多 态性，占总数的 82.29 %，表明西葫芦种质资源在分子水平上具有丰富的遗传多样性，与形态学研究结果一 致。聚类分析将 47 份西葫芦种质材料分为两大类。在第Ⅰ类群中，可将 32 份种质分为 4 个亚类；第Ⅱ类 群分成 5 个亚类。从生长习性来看，矮生与半蔓生的亲缘关系最近；从皮色来看，白色与绿色亲缘关系最 近，亲缘关系最远的为白色与花皮。 关键词：西葫芦；种质；遗传多样性；RAPD 中图分类号：S642.6 文献标识码：A 文章编号： 1000-6346（2010）16-0026-06 Analysis of Genetic Diversity in Summer Squash Germplasm by RAPD 1 2* CHEN Feng-zhen , HE Qi-wei 1 2 （Department of Landscape Engineering, Heze University, Heze 274000, Shandong, China; Vegetable Research Institute, Shandong Academy of Agricultural Sciences, Ji’nan 250100, Shandong, China ） Abstract ：In this study, RAPD analysis was applied to assess the genetic diversities and relationship with 47 internal and external summer squash（ Cucurbita pepo L. ）germplasm resources. Genomic DNA of 47 summer squash germplasm resources was amplified by RAPD. 30 polymorphic primers screened out of 520 random primers were used and 367 reproducible bands were produced. Among them, 302 bands （82.29 %）were polymorphic. The genetic diversity of summer squash germplasm resources was very rich. This result is consistent with morphological study. The cluster analysis divided the germplasm resources used in this study into 2 groups. The first group was divided into 4 sub-groups. The second group was divided into 5 sub-groups. The different sub-groups of summer squas