地塞米松诱导大鼠白内障形态学及酶活性变化论文.docVIP

　　地塞米松诱导大鼠白内障形态学及酶活性变化论文 王建伟，严宏，王永强，哈文静，丁正华，郭勇 【关键词】 白内障 Alteration of morphology and enzyme activity in dexamethasoninduced cataract in rat lens 【Abstract】 AIM: To observe the morphological features and enzyme activities in dexamethasoninduced organcultured rat lens and to study the mechanisms of steroid cataract. METHODS: One hundred lenses cultured in vitro ethasoninduced cataract group (DMEM+dexamethason 10 μmol/L). Folloicroscope. On d 1, 3, 5 and 7, 12 lenses of each group ogenized and the activities of enzymes (catalase, superoxide dismutase, lactate dehydrogenase) easured, respectively. Tade into specimen of transmission electron microscopy for the observation of the ultrastructure on d 7. RESULTS: The lenses of control group exhibited mistlike opacity and those of cataract group had dense nuclear opacity on d 7. On d 3, the activities of catalase, superoxide dismutase and lactate dehydrogenase decreased by 34.51%, 7.78%, 15.73% respectively pared icroscope, the arrangement of fiber cells in control group embrane, cell junction and organelle al, ent of fiber cells alappearing cells itochondria, a large amount of vacuoles and expanded extracellular lacunae. CONCLUSION: Lenses have nuclear cataract in dexamethasoninduced organcultured rat lens. Oxidative stress may be involved in the steroidinduced cataract formation. 【Keyutase; lactate dehydrogenase; transmission electron microscope 【摘要】 目的：观察地塞米松诱导离体大鼠激素性白内障的形态学和酶活性变化，探讨激素性白内障发病机制. 方法：大鼠的100只透明晶状体随机分为对照组（DMEM）、地塞米松诱导的白内障组（DMEM＋地塞米松10 μmol/L），体外培养7 d.freela公司产品，CAT,SOD,LDH测试盒购自南京建成生物工程研究所. 1.2方法 1.2.1离体晶状体培养以颈椎脱臼法处死SD大鼠，取其眼球，剔除肌肉、筋膜等组织，安尔碘浸泡5 min，生理盐水冲洗3次，将眼球浸于DMEM液中，由后极部剪开巩膜，取出晶状体. 尽量去除晶状体表面的玻璃体. 将晶状体放入含5×104 u/L青霉素和5×104 u/L链霉素的DMEM培养基中，于37℃下，置50 mL/L CO2细胞培养箱中培养8 h后，弃去混浊晶状体，留取100只透明晶状体备用. 1.2.2激素性白内障模型的建立［5］将100只备用晶状体按随机数字大小等分为两组：正常对照组（A:DMEM），地塞米松诱导的激素性白内障组（B:DMEM+地塞米松10 μmol/L）. 分别放入含培养基的无菌24孔板中，于37℃下，置50 mL/L CO2细胞培养箱中培养，隔日换液. 培养中每日用解剖显微镜观察晶状体，记录晶状体混浊程度. 各组分别于第1,3,5,7日取出12只晶状体，称质量，-70℃冰箱冷冻保存.