多维在线测定生物体液中碱性药物.pdfVIP

Multidimensional On-Line SPE for Undisturbed LC-MS-MS Analysis of Basic Drugs in Biofluids 2006, 63, 523–531 K. Georgi, K.-S. Boos Laboratory of BioSeparation, Institute of Clinical Chemistry, University Hospital, 81366, Munich, Germany; E-Mail: boos@med.uni-muenchen.de Received: 31 March 2006 / Revised: 13 April 2006 / Accepted: 17 April 2006 Online publication: 31 May 2006 analysis, i.e. therapeutic drug monitoring Abstract (TDM). However, despite of the high selectiv- In bioanalytical LC-MS-MS matrix effects influencing the ionization process are a major concern ity and specificity of modern mass with respect to the quality of the results obtained. In general such matrix effects are directly spectrometers the analysis of highly related to an insufficient sample clean-up of the biofluids. In order to establish a MS-adequate complex samples such as blood, plasma clean-up procedure for basic analytes present in biofluids (e.g. urine, plasma) which is based and urine is often error prone. The main on solid-phase extraction (SPE) principles a combination of tailor-made SPE column packings reasons for incorrect analyses are so and automated column-switching was developed. This novel, multidimensional (MD) SPE called matrix effects. They are the result platform relies on the combination of a SPE column packed with a restricted access material of a change in the ionization efficiency of (RAM) allowing size-exclusion and re