冬虫夏草菌CS.SYSU-Ⅱ深层发酵条件的优化.pdfVIP

2016 Vo l.35 NO.5 • 70 • Serial NO.291 China Brewing Research Report 冬虫夏草菌CS.SYSU-II 深层发酵条件的优化 盛嘉俊，陈晓光，叶克难* (中山大学生命科学学院，广东广州 510275) 摘 要:以菌丝体干质量为评价指标，对冬虫夏草菌(Cordyceps sinensis) CS.SYSU- II 在5L发酵罐中的发酵条件进行优化。在单因素 试验基础上，选取搅拌转速、通气量和装料系数，采用3 因素3水平的正交设计试验，得出最佳发酵条件并验证，最后扩大至20 L发酵 罐。结果表明，冬虫夏草菌CS.SYSU-II 在5L发酵罐中培养6d的最佳发酵条件为搅拌转速220 r/min、通气量2.0 vvm和装料系数60%， 在此条件下进行验证试验，菌丝体干质量能达到(19.85:1: 0.52) glL。扩大至20 L发酵罐，可得菌丝体干质量为(16.78:1: 0.06) glL 发酵结 果能达到5L发酵罐的85%。通过比较摇床与5L发酵罐的生长曲线，发现pH值能反映菌丝体干质量的变化趋势，可将pH值的变化规 律作为发酵结束的判断依据。 关键词:冬虫夏草菌:深层发酵:菌丝体:发酵条件:优化 中图分类号:Q815 文章编号:0254-5071(2016)05-0070-05 doi:10.11882/j .issn.0254-5071.2016.05.015 Optimization of submerged fermentation conditions of Cordyceps sinensis CS.SYSU- II SHENG Jiajun, CHEN Xiaogua吨， YE Kenan* (CoJJege ofLife Science, Sun Yat-sen University, Guangzhou 510275, China) Abstract: Using the dry mass ofmycelia as evaluation index , the fermentation conditions of Cordyceps sÍnensís CS.SYSU- II in 5 L fermenter were optimized. 8ased on single factor experiments, stirring speed, aeration rate and volume charge coefficient were selected. The optimum fermentation conditions were determined by 3 factors and 3 levels orthogonal experiments, then verified , and finally extended to 20 L fermenter. The results showed that the optimum conditions of C. sínensís CS.SYSU- II in 5 L fermenter fermented for 6 d were stirring speed 220 r/min , aeration rate 2.0 智vm and volume charge coefficient 60%. Under the conditions , the dry mass ofmycelia could reach (19.85 :1: 0.52) gIL. The dry mass ofmycelia in 20 L fermenter was (1 6.78:1: 0.06) 衅， which could reach 85% of that in 5 L f