无机氮源对出芽短梗霉发酵普鲁兰多糖分子量的影响.PDFVIP

现代食品科技 Modern Food Science and Technology 2017, Vol.33, No.5 无机氮源对出芽短梗霉发酵普鲁兰多糖分子量的 影响 安超，马赛箭，薛文娇，上官亦卿，丁浩 （陕西省微生物研究所，陕西西安 710043） 摘要：以出芽短梗霉（Aureobasidium pullulans ）CGMCC No.11062 为出发菌株，研究五种无机氮源对普鲁兰多糖产量、结构、 纯度及分子量的影响。结果表明：无机氮源种类对普鲁兰多糖产量和分子量产生显著影响，未影响普鲁兰多糖结构和纯度。其中，以 硫酸铵（1.5 g/L ）为唯一氮源时普鲁兰多糖产量达到32.84 g/L，重均分子量（Weight-average Molecular Weight ，Mw ）最大，为799823 ku。硫酸铵浓度对普鲁兰多糖的产量和分子量影响显著，而未显著影响普鲁兰多糖结构。随着硫酸铵浓度的增加，普鲁兰多糖产量和 分子量同时增加；当硫酸铵浓度为 1.5 g/L 时，普鲁兰多糖的产量出现最大值，为32.45 g/L；而当硫酸铵浓度为2.1 g/L ，普鲁兰多糖 重均分子量（Mw ）最大，达到1236958 ku。所有制得的普鲁兰多糖纯度在 95%~99%之间。这些研究可为不同分子量普鲁兰多糖生 产提供技术指导。 关键词：出芽短梗霉；普鲁兰多糖；分子量；无机氮源；重均分子量 文章篇号：1673-9078(2017)5-213-219 DOI: 10.13982/j.mfst.1673-9078.2017.5.034 Effects of Inorganic Nitrogen Sources on the Molecular Weight of Pullulan Produced by Aureobasidium pullulans AN Chao, MA Sai-jian, XUE Wen-jiao, SHANGGUAN Yi-qing, DING Hao (Microbiology Institute of Shanxi, Xi’an 710043, China) Abstract: The effects of five types of inorganic nitrogen sources on the yield, structure, purity, and molecular weight of pullulan were studied using Aureobasidium pullulans CGMCC No. 11062 as the parental strain. The results showed that the yield and molecular weight of pullulan were significantly influenced by the types of inorganic nitrogen source, whereas the structure and purity of pullulan were not affected. When ammonium sulfate (1.5 g/L) was used as the sole nitrogen source, the yield of pullulan was 32.84 g/L, and the maximum weight-average mol