突触后钙通路有助于视网膜视锥和L型水平细胞之间的突触可塑性.pdfVIP

Acta Physiologica Sinica , October 25, 2006, 58 (5): 407-414 407 Research Paper Postsynaptic calcium pathway contributes to synaptic plasticity between retinal cones and luminosity-type horizontal cells 1 1, ** 2 2,* HUANG Shi-Yong , HU Jian-Feng , GONG Hai-Qing , LIANG Pei-Ji 1 2 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Department of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China Abstract: It was previously found that the efficacy of synaptic transmission between retinal cone systems and luminosity-type horizontal cells (LHCs) was activity-dependent. Repetitive activation of red-cone pathway increased the LHC’s hyperpolarizing response to red light, and the response enhancement was reversible. In this study, intracellular recording and pharmacological method were applied to investigate the mechanism(s) underlying red-flickering-induced response enhancement. Lowering intracellular Ca2+ in the LHC by intracellular injection of Ca2+ chelator EGTA prevented the development of red-flickering-induced response enhancement, which implicates the importance of postsynaptic calcium signal. The response enhancement could also be eliminated by a potent antagonist of Ca2+-permeable AMPA receptor (CP-AMPAR), which suggests the possibility that Ca2+ influx via glutamate-gated calcium channels is related to the changes of [Ca2+] . Furthermore, the administration of ryanodine or caffeine also attenuated the i phenomenon, which gives evidence that the local calcium signal caused by intracellular calcium-induced calcium release (CICR) may be involved. Taken to